We narrowed to 13,953 results for: LEA
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Plasmid#216283PurposeExpresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.DepositorInsertSpyCatcher003-TEVs-SpyTag003DA
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1553 - pAAV EF1a EGFP-KASH
Plasmid#131682PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFPDepositorInsertEGFP-KASH
UseAAVTagsKASHAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH1-shOPA1-pSyn-tdtomato
Plasmid#206316PurposeKnocking-down OPA1 in mouse cellsDepositorInsertshRNA against mouse OPA1
UseLentiviralExpressionMammalianAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT2h
Plasmid#208721PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseAAVPromoterhSynAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF mito-cGFP (matrix)
Plasmid#188904PurposeExpresses mitochondrial GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmitochondrial GFP
TagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO_Ollas.S.V5_mCherry-NLS
Plasmid#178211PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsNuclear Localization Signal and Ollas.S.V5PromotereF1aAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ad:2CMV_A2R2-N25K3
Plasmid#122242PurposeExpresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backboneDepositorUseAdenoviralTagsSignal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…ExpressionMammalianPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Right DdCBE-G1397-C-wildtype
Plasmid#179687PurposeExpression of base editor in mammalian cellsDepositorInsertSOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR
ExpressionMammalianAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDTFendoNA2
Plasmid#174731PurposeExpresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.DepositorInsertDT386F
ExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
Tags6XHisExpressionBacterialMutationnonePromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-MBP-4xDQNAT
Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046230
Plasmid#145454PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a His6Flag2AUF1(p42)
Plasmid#135941PurposeExpresses AUF1p42 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-CXCR4
Plasmid#133431PurposeHuman CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertCXCR4 (CXCR4 Human)
ExpressionBacterialAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH111gp160
Plasmid#123268PurposeMammalian expression plasmid for Env from the CH111 HIV-1 isolateDepositorInsertHIV-1 (AD8) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JAK3 (JAK3A-c023)
Plasmid#98229PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJAK3A (JAK3 Human)
UseBaculovirusTagsHis-6-TEVMutationpfam00069:Pkinase 822-1091PromoterpolyhedrinAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ninein coiled-coil 2
Plasmid#73522PurposeEncodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertninein coiled-coil 2 (Nin Mouse)
TagsEGFP tagExpressionMammalianPromoterCMVAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ninein coiled-coil 1
Plasmid#73521PurposeEncodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertninein coiled-coil 1 (Nin Mouse)
TagsEGFP tagExpressionMammalianPromoterCMVAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only