We narrowed to 15,732 results for: LEA

1. 6599-SUMO1GG

   Plasmid

   #13377

   Depositor

   Anne Dejean

   Article

   Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

   Insert

   SUMO1 (SUMO1 Human)
   

   Expression

   Mammalian

   Mutation

   mature form ending with GG

   Available Since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Tol2-CAG::Nucbow

   Plasmid

   #158992

   Purpose

   Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Nucbow
   

   Tags

   All FPs are fused to H2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SpCas9-NRTH

   Plasmid

   #198541

   Purpose

   Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRTH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-Ahi1

   Plasmid

   #30494

   Depositor

   Joseph Gleeson

   Article

   Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

   Insert

   Ahi1 (AHI1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV_NOPlight1

   Plasmid

   #195578

   Purpose

   Expresses NOPLight1 in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

   Insert

   NOPLight1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSLS.419

   Plasmid

   #187208

   Purpose

   Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco4 ncRNA
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

   Plasmid

   #176587

   Purpose

   Bacterial expression of His14-MBP-TEV-AmaTnpB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Inserts

   MBP tag
   TnpB
   

   Tags

   His14 tag

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-VCPIP1

   Plasmid

   #85671

   Purpose

   Expression of VCPIP1 with N-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   VCPIP1 (VCPIP1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Lyso-mCherry-Rheb(C181S)

   Plasmid

   #192438

   Purpose

   Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   Lyso-mCherry-Rheb(C181S) (RHEB Human)
   

   Tags

   Full-length LAMP1 and mCherry

   Expression

   Mammalian

   Mutation

   Rheb Cys 181 mutated to Ser; abolishes C-terminal…

   Promoter

   CMV

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA6A-EGFR ICD (645-1186)

    Plasmid

    #42667

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR ICD (645-1186) (EGFR Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pDisplay-GRAB_g5-HT2h

    Plasmid

    #208709

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT2h
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TUBG1

    Plasmid

    #87954

    Purpose

    expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein

    Depositor

    Maria Alvarado-Kristensson

    Article

    Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    whole protein, The C-terminal is a sh-gamma-tubul…

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

    Plasmid

    #122083

    Purpose

    Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-LaminB1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of LaminB1

    Expression

    Bacterial and Mammalian

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Akt-STOPS-NES

    Plasmid

    #175008

    Purpose

    Cytosol-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

    Plasmid

    #169921

    Purpose

    DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

    Depositor

    Zhili Rong

    Article

    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

    Insert

    IRES-Puro-pEF1a-EGFP
    

    Use

    CRISPR

    Promoter

    EF1a

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CS2-Myc-hASUN

    Plasmid

    #47955

    Purpose

    Expression of tagged-human ASUN

    Depositor

    Laura Lee

    Article

    Jodoin et al Mol Biol Cell. 2013 Jul 31.

    Insert

    Asunder (INTS13 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    Sp6

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. SpCas9-NG-CGBE1

    Plasmid

    #198537

    Purpose

    Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSOMA-NLS

    Plasmid

    #118937

    Purpose

    Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-WT (AF41)

    Plasmid

    #21367

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205320

    Purpose

    All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAba30 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge