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Plasmid#164423Purposesingle guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDarmo.CMVT_v1
Plasmid#133072PurposeEntry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.DepositorTypeEmpty backboneUseGateway CloningTags3C-Flag-TwinStrep and TwinStrep-Flag-3CExpressionMammalianPromoterCMVT (Enhanced CMV)Available SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Htr5b(3.7)-tTA-sv40
Plasmid#184412PurposeAAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.DepositorInsertMouse Htr5b gene promoter-tTA
UseAAVTagstTAExpressionMammalianPromoterMouse Htr5b geneAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest iRFP
Plasmid#62176PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorExpressionMammalianAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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meffBlue chromoprotein
Plasmid#117845PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, meffBlue
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS N terminal HA
Plasmid#217639PurposeEntry vector with WT1 +KTS mutant with N terminal HA for gateway cloningDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 -KTS N terminal HA
Plasmid#217641PurposeEntry vector with WT1 -KTS mutant with N terminal HA for gateway cloningDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-NES-L2
Plasmid#127351Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway CloningTagsGS linker, NES, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75154PurposeNuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
cjBlue chromoprotein
Plasmid#117844PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, cjBlue
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-SpCas9-BlastR
Plasmid#107190PurposeConfers constitutive expression of SpCas9DepositorInsertSpCas9 (NEWENTRY )
UseCRISPRTags3xFLAGExpressionBacterial and MammalianPromoterChicken ß-actinAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV Renilla Luciferase L5-L2
Plasmid#62186PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloningDepositorInsertRenilla luciferase
UseGateway Cloning and LuciferaseExpressionMammalianPromoterCMVAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-NES-YFP-STOP-L2
Plasmid#127358Purposegateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway CloningTagsGS linker, NES, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-B/c
Plasmid#227962PurposeEntry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.DepositorInsertB/c
PromoterNoneAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
meffRed chromoprotein
Plasmid#117836PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coliDepositorInsertpromoter, RBS, meffRed
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PLXNB2
Plasmid#86236PurposePLXNB2 full-length human cDNA CDS in gateway entry plasmidDepositorInsertPLXNB2 (PLXNB2 Human)
UseGateway CloningAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR_sfGFP-TurboID
Plasmid#240226PurposeGateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)DepositorInsertsfGFP-TurboID
UseGateway shuttle vectorAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A3
Plasmid#132160PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A3 (SLC6A3 Human)
ExpressionMammalianAvailable SinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN_TT 3xFLAG-CSE1L
Plasmid#192295PurposeGateway entry vector for an inducible 3xFLAG-CSE1LDepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only