10,100 results

1. pTet-On-TAP

   Plasmid

   #59017

   Purpose

   Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.

   Depositor

   David Sibley

   Article

   Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

   Type

   Empty backbone

   Use

   Toxoplasma expression

   Tags

   Calmodulin Binding Peptide fused with a TEV Cleav…

   Expression

   Mutation

   Promoter

   RPS13 with Tet Operators Inserted

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPB-EF1a-MegaGate-HA-Hygro

   Plasmid

   #175268

   Purpose

   MegaGate destination with C-terminal HA tag and hygromycin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PJQ47

   Plasmid

   #134646

   Purpose

   Contains Promoter 1 with bsa1 cut sites for inserting sgRNA

   Depositor

   Oren Rosenberg, Jason Peters

   Article

   Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson BBa_J23117

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PJQ48

   Plasmid

   #134647

   Purpose

   Contains Promoter 2 with bsa1 cut sites for inserting sgRNA

   Depositor

   Oren Rosenberg, Jason Peters

   Article

   Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson BBa_J23114

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOSV809

   Plasmid

   #126602

   Purpose

   modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

   Depositor

   Jean-Luc Pernodet

   Article

   Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHLmMBP-11

   Plasmid

   #72349

   Purpose

   Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGW-TurboID-GOI-GFP

   Plasmid

   #209383

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET His10 TEV LIC cloning vector (2B-T-10)

   Plasmid

   #78173

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   10xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

   Plasmid

   #182352

   Purpose

   Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG423GAL-ccdB

    Plasmid

    #14149

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge