165,567 results

1. pMIH-TOMM20-Halo

   Plasmid

   #111626

   Purpose

   Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

   Depositor

   Benjamin Kile

   Article

   McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

   Insert

   TOMM20 (Tomm20 Mouse)
   

   Use

   Retroviral

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-HA-USP18

   Plasmid

   #22572

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP18 (USP18 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKH3-TRIM28

   Plasmid

   #45569

   Depositor

   Fanxiu Zhu

   Article

   Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

   Insert

   TRIM28 (TRIM28 Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. mEGFP-NbALFA

   Plasmid

   #159986

   Purpose

   For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mEGFP-NbALFA
   

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mEGFP and NbALFA

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-EcoRIR

   Plasmid

   #165504

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   EcoRI
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEGFP-C9orf72

   Plasmid

   #83437

   Purpose

   Expresses GFP-C9orf72 in mammalian cells

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   C9orf72 (C9orf72 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. FHRE-Luc

   Plasmid

   #1789

   Purpose

   Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FHRE (FOXO3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   3 copies of FRE

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICS Mt-ER- PM Long P2A

   Plasmid

   #164109

   Purpose

   Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP PM-Mt-ER Long P2A
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMma PylT_GFP 150TAG 6xHis

   Plasmid

   #140015

   Purpose

   Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LRCherry2.1

    Plasmid

    #108099

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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