We narrowed to 6,761 results for: SIM;

1. pGEX6P1-hcGAS(157-522)

   Plasmid

   #108676

   Purpose

   For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

   Insert

   cGAS amino acid 157-522 (CGAS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acid 157-522

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-FFSS-Cyclin D1

   Plasmid

   #215151

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-TetOne-FFSS-Cyclin D1

   Plasmid

   #215146

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-FFSS-Cyclin D1 (T286A)

   Plasmid

   #215152

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   With T286A mutation.

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIPZ-p21-HA

   Plasmid

   #215110

   Purpose

   Expresses p21-HA in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (Tet-inducible)

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1e-CDK9/Cyclin T1

   Plasmid

   #231732

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CDK9 (CDK9 Human)
   CCNT1 (CCNT1 Human)
   

   Use

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB2

   Plasmid

   #195282

   Purpose

   Bicistronic DTR and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR 2A Puro (HBEGF Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. R703-M72-303: CMV51p> Halotag-Hs.KRAS4b

   Plasmid

   #159679

   Purpose

   Mammalian protein expression of KRAS4b-Halotag fusion

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Insert

   Halotag-Hs.KRAS4b (KRAS Human)
   

   Use

   Tags

   Halotag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. R713-M88-303: CMV51p> Halotag-Hs.HRAS

   Plasmid

   #159681

   Purpose

   Mammalian protein expression of HRAS-Halotag fusion

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Insert

   Halotag-Hs.HRAS (HRAS Human)
   

   Use

   Tags

   Halotag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFB2A

    Plasmid

    #195283

    Purpose

    Bicistronic DTR and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Hygro (HBEGF Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. R713-M91-303: CMV51p> Halotag-Hs.NRAS

    Plasmid

    #159682

    Purpose

    Mammalian protein expression of NRAS-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.NRAS (NRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Beta-2-adrenergic receptor-YFP

    Plasmid

    #67146

    Purpose

    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1_c-SRC

    Plasmid

    #171850

    Purpose

    pEGFP-N1_c-SRC

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    c-SRC (SRC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Added a linker sequence in 3' of c-SRC: GSGS

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBIG1e-CDK12/Cyclin K

    Plasmid

    #231730

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBIG1e-CDK13/Cyclin K

    Plasmid

    #231731

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK13 (CDK13 Human)
    CCNK (CCNK Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p21-HA(RxL)-NeoR

    Plasmid

    #215106

    Purpose

    Expresses p21-HA(RxL) in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mNeonGreen-LIG3

    Plasmid

    #215143

    Purpose

    Expresses mNeonGreen-LIG3 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    LIG3 (LIG3 Human)
    

    Use

    Retroviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

    Plasmid

    #107307

    Purpose

    Encoding an SPH activation system

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-SOUL-P2A-tdTomato

    Plasmid

    #153019

    Purpose

    Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    P2A-tdTomato

    Expression

    Mammalian

    Mutation

    C128S/D156A/T159C

    Promoter

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid

    #216155

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic splice site and C-terminal FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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