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V622 pGEX-2TK-LP - Acceptor

Plasmid

#11716

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

Plasmid

#62954

Purpose

Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70

Plasmid

#62911

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pWZL blast goosecoid

Plasmid

#14095

Depositor

Bob Weinberg

Article

Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

goosecoid (Gsc Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pKT0146

Plasmid

#8742

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

tdimer2

Tags

Codon optimized linker

Expression

Yeast

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

Plasmid

#122085

Purpose

Express M.EcoGII fused to CENPC with destabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)

Use

Retroviral

Tags

V5 tag on Nter of CENPC

Expression

Bacterial and Mammalian

Mutation

L341F, R930L (see comments below)

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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HOXA9-MSCV-CD24

Plasmid

#20975

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

Inserts

HOXA9 (Hoxa9 Mouse)
CD24 (CD24 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Full length cDNA with ~1.1kb 3'UTR with 2 pu…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP PR-D25A

Plasmid

#101334

Purpose

pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 envFS

Use

Lentiviral

Mutation

Protease D25A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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TAL-L1-1.3-KRAB

Plasmid

#103034

Purpose

[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domain

Depositor

Maria-Elena Torres-Padilla

Article

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3-KRAB

Use

T3 in vitro transcription for expression in mamma…

Tags

HA and KRAB

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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TAL-L1-2.4-VP64

Plasmid

#103039

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

Depositor

Maria-Elena Torres-Padilla

Article

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-2.4-VP64

Use

T3 in vitro transcription for expression in mamma…

Tags

HA and VP64

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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TAL-L1-1.3-VP64

Plasmid

#103033

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

Depositor

Maria-Elena Torres-Padilla

Article

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3-VP64

Use

T3 in vitro transcription for expression in mamma…

Tags

HA and VP64

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CRY2-CreN

Plasmid

#75268

Purpose

The second half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)

Use

AAV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

Plasmid

#62953

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
IVT-nCas9(H840A)

Plasmid

#207455

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

H840A

Expression

Bacterial

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIIA

Plasmid

#186825

Purpose

Expresses FLAG-CaMKIIA in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2a (Camk2a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHY002_Strep_Avi_hsDCAF15

Plasmid

#174479

Purpose

Expresses DCAF15 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DCAF 15 (DCAF15 Human)

Tags

StrepII-Avi-TEV

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1E

Plasmid

#160442

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b V12

Plasmid

#118323

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b V12 (RAP1B Bovine)

Tags

Flag

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pICH51288

Plasmid

#50269

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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