19,810 results

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NES-EGFP-cPHx3

Plasmid

#116855

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMIG-hKLF4

Plasmid

#17227

Depositor

George Daley

Article

Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Insert

Kruppel-like factor 4 (gut) (KLF4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Article

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-Ascl1

Plasmid

#27150

Depositor

Marius Wernig

Article

Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mCherry from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD1

Plasmid

#33109

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD1 (TEAD1 )

Tags

Myc

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9

Plasmid

#75381

Purpose

dCas9

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Sp dCas9

Use

Lentiviral

Tags

no

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-DDB1

Plasmid

#19918

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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LeGO-V2

Plasmid

#27340

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Venus fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pBB542

Plasmid

#27395

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ, groESL

Expression

Bacterial

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pSBtet-BP

Plasmid

#60496

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM4D(Gi)-mCherry

Plasmid

#50477

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has Service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM4D(Gi)-mCherry (CHRM4 Human)

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Short P2A

Plasmid

#164110

Purpose

Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM- Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab9a

Plasmid

#78592

Purpose

Express mCherry-Rab9a in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

Rab9a (RAB9A Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-2NLS-Cas9-6XMS2

Plasmid

#166033

Purpose

Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

SpCas9

Use

CRISPR and Lentiviral

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-H

Plasmid

#66187

Purpose

Expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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YFP-CL1

Plasmid

#11950

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

YFP-CL1

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pDA083

Plasmid

#131389

Purpose

eGFP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

eGFP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-DYRK1B

Plasmid

#23761

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK1B (DYRK1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHex

Plasmid

#124670

Purpose

Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHex

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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