We narrowed to 9,184 results for: Pol;

1. GfaABC1D BARK D110A p2A mCherry

   Plasmid

   #117692

   Purpose

   GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   AAV

   Tags

   HA / FLAG

   Mutation

   D110A

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #71236

   Purpose

   Express sgRNA and dCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A Puro
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Mutation

   D10A and H840A

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Expression

   Mammalian

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP339 pGK-dCas9-SunTag

   Plasmid

   #100943

   Purpose

   dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Src-Y530F

   Plasmid

   #124659

   Purpose

   Human c-Src with Y530F mutation

   Depositor

   Cheng-han Yu

   Article

   Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

   Insert

   cSrc (SRC Human)
   

   Expression

   Mammalian

   Mutation

   Y530F

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS1994

   Plasmid

   #49124

   Purpose

   AAV plasmid with S100B (Ple266) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple266-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   S100B

   Available Since

   May 6, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLentiDP1.0

   Plasmid

   #171094

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-dCas9-HA

   Plasmid

   #61355

   Purpose

   encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-Axin1

   Plasmid

   #109370

   Purpose

   Transient over-expression of human Axin1

   Depositor

   Mariann Bienz

   Article

   Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

   Insert

   Axin1 (AXIN1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. hGFAP-GFP

    Plasmid

    #40592

    Depositor

    Albee Messing

    Article

    Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.

    Insert

    GFP-S65T
    

    Use

    Mouse Targeting

    Tags

    MP-1 fragment (mouse protamine 1 intron and polya…

    Expression

    Bacterial and Mammalian

    Mutation

    Construct contains a mutated, humanized hGFP(S65T…

    Promoter

    hGFAP promoter

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEos3-Rac1

    Plasmid

    #92039

    Purpose

    Expresses mEos3-Rac1 fusion protein

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEos3-dnRac1

    Plasmid

    #92040

    Purpose

    Expresses mEos3 fusion with dnRac1 (T17N).

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T17N (dominant negative)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4_NFAT-RE-CMVmin-BC0524-luc2

    Plasmid

    #227139

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC0524

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEMS2159

    Plasmid

    #70120

    Purpose

    Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-MCS-iCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    May 12, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. peGFP C2-IST1

    Plasmid

    #218582

    Purpose

    Expresses GFP-tagged human IST1 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    IST1 (IST1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. W118-1_hTGM2-Flag

    Plasmid

    #180407

    Purpose

    lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

    Plasmid

    #140892

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F-mCherry (tpte, vsp Zebrafish, Ciona intestinalis)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE)

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Str-KDEL_SBP-mCherry-CCR1

    Plasmid

    #222311

    Purpose

    Synchronize the trafficking of CCR1 from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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