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pCSC-NGN2-IRES-ISL1-T2A-LHX3

Plasmid

#221814

Purpose

Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

Depositor

Baojin Ding

Article

Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

Inserts

NEUROG2 (NEUROG2 Human)
ISL1 (ISL1 Human)
LHX3 (LHX3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2-356bp

Plasmid

#16292

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -344 to +12 relative to first transcript…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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EF1a_HNF1A_P2A_Hygro_Barcode

Plasmid

#120448

Purpose

Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HNF1A (HNF1A Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau

Plasmid

#46904

Purpose

expresses EGFP tagged Tau in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

This WT htau construct contains human four-repeat…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-pHluorin_M153R-CD63-mScarlet

Plasmid

#172118

Purpose

Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

mScarlet and pHluorin_M153R in a small extracellu…

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG-GATA2

Plasmid

#1418

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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HNF1B_pLX307

Plasmid

#98342

Purpose

Lentiviral expression of HNF1B

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HNF1B (HNF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Rtn3LΔ6LIR-mNeonGreen

Plasmid

#169036

Purpose

Express human Rtn3L with 6x LC3-interacting regions mutated in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3L (RTN3 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

Changed following LC3-interacting regions of Rtn3…

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-pro-sumo1

Plasmid

#25101

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

Changed Serine 2 to Alanine

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PFKM

Plasmid

#23728

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKM (PFKM Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pET-15b-hAR-663-919

Plasmid

#89083

Purpose

bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR ligand binding domain (AR Human)

Tags

His

Expression

Bacterial

Mutation

aa 663-919

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNASE1_Exo-endo-phos

Plasmid

#110004

Purpose

Protein expression and purification of DNASE1_Exo-endo-phos

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNASE1_Exo-endo-phos (DNASE1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G129E

Plasmid

#81689

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

G129E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST EGFP-flag-CAD

Plasmid

#188118

Purpose

Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

EGFP tag and Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDUAL CLDN (GFP)

Plasmid

#86981

Purpose

Lentiviral expression construct encoding Claudin-1 and GFP from separate promoters

Depositor

Joe Grove

Insert

CLDN1 (CLDN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV/hPGK

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Occludin-C-14

Plasmid

#54211

Purpose

Localization: Tight Junctions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Occludin (OCLN Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-PFKL

Plasmid

#23729

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKL (PFKL Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)

Plasmid

#228043

Purpose

DONR vector for inflammasome speck reporter, designed from PMC9623368.

Depositor

Wade Harper

Insert

CASP1[CARD]-mSG2

Use

Gateway donr

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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p11U1ADb

Plasmid

#27384

Depositor

David Bartel

Article

Shechner et al Science. 2009 Nov 27. 326(5957):1271-5.

Insert

U1A A1-98 Y31H/Q36R (SNRPA Human)

Expression

Bacterial

Mutation

A1-98 Y31H/Q36R double mutant

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
pGL2-200bp

Plasmid

#16293

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -188 to +12 relative to first transcript…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only