We narrowed to 15,742 results for: grna

1. LENTICRISPR-GPI_sgRNA2

   Plasmid

   #201595

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   GPI (GPI Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA5_8xCTS-ECFP-pA

   Plasmid

   #200247

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 5

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA5_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA3_8xCTS-ECFP-pA

   Plasmid

   #200245

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 3

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA3_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA2_8xCTS-ECFP-pA

   Plasmid

   #200244

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 2

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA2_8xCTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pegRNA_HEK4_1bp-insertion

   Plasmid

   #201977

   Purpose

   Express a pegRNA used for 1bp insertion at HEK4 site

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   pegRNA_HEK4_1bp-insertion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AV13_pCAG.Cas9.gRNA.NT

   Plasmid

   #199260

   Purpose

   Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   Non-targeting (NT) control gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRa_CHO-Bip_gRNA1

   Plasmid

   #183694

   Purpose

   CRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   gRNA targeting chinese hamster Bip (Hspa5)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

   Plasmid

   #197963

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-vCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 and pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC1300_pUB10_pco-nCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197980

   Purpose

   T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-nCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCVp and pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Chr1q_Cassette-Proximal_gRNA

    Plasmid

    #195127

    Purpose

    gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr1q gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPRv2_ZsG gRNA_4

    Plasmid

    #192688

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2_ZsG gRNA_1

    Plasmid

    #192685

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiCRISPRv2_ZsG gRNA_2

    Plasmid

    #192686

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPRv2_ZsG gRNA_3

    Plasmid

    #192687

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPgiCas13b-gRNA-1

    Plasmid

    #184834

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPgiCas13b-gRNA-NT

    Plasmid

    #184835

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLQ1373_mU6_sgRNA targeting e1

    Plasmid

    #190684

    Purpose

    sgRNA targeting enhancer 1 of MYC

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNA targeting enhancer 1 of MYC
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. double_sgRNA targeting e1 and e4

    Plasmid

    #190688

    Purpose

    sgRNAs targeting enhancer 1 and 4 of MYC separately

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNAs targeting enhancer 1 and 4 of MYC separately
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ5004_hU6_sgRNA targeting e7

    Plasmid

    #190687

    Purpose

    sgRNA targeting enhancer 7 of MYC

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNA targeting enhancer 7 of MYC
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ5004_hU6_sgRNA targeting e4

    Plasmid

    #190686

    Purpose

    sgRNA targeting enhancer 4 of MYC

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNA targeting enhancer 4 of MYC
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-v2-ALDH1A3gRNA2

    Plasmid

    #189747

    Purpose

    The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    Cas9, ALDH1A3 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-ALDH1A3gRNA3

    Plasmid

    #189748

    Purpose

    The construct was used for expression of gRNA for knocking out of the ALDH1A3 gene in Cas9 expressing cells.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A3 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-v2-ALDH1A3gRNA1

    Plasmid

    #189746

    Purpose

    The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    Cas9, ALDH1A3 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSLQ1373_mU6_sgRNA targeting e3

    Plasmid

    #190685

    Purpose

    sgRNA targeting enhancer 3 of MYC

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNA targeting enhancer 3 of MYC
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPbuCas13b-gRNA-1

    Plasmid

    #184567

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPlacPbuCas13b-gRNA-NT

    Plasmid

    #184838

    Purpose

    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Sp(AU)sgRNA-mKO2

    Plasmid

    #184443

    Purpose

    sgRNA with AU flip (sgRNA2.0), mKO2 as selection marker

    Depositor

    Rhys Allan

    Article

    Chan et al Immunol Cell Biol. 2020 Sep 24. doi: 10.

    Insert

    sgRNA with AU flip, mKO2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid

    #178096

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + MTOR nick exon-44 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid

    #178099

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + MTOR nick intron-45 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid

    #178105

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 + MTOR nick exon-44 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge