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We narrowed to 886 results for: nanobody

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Showing: 841 - 870 of 886 results

  1. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid
    #112157
    Purpose
    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.
    Depositor
    Ben Giepmans
    Article
    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261
    Insert
    anti-GFP FLIPPER-body
    Tags
    His and Thrombin cleavage site
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112157-plasmid-map-sequence-id-213698

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  2. pmEos3.1-N1 GBP

    Plasmid
    #220492
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Frederic Meunier
    Article
    Gormal et al Nat Protoc. 2025 Aug 8. doi: 10.1038/s41
    Insert
    GFP Binding Protein tagged with mEos3.1
    Tags
    mEos3.1
    Expression
    Mammalian
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    220492-plasmid-map-sequence-id-442019

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  9. EGFP–COSMO–PB

    Plasmid
    #169684
    Purpose
    COSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104W
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169684-plasmid-map-sequence-id-338138

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  10. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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  11. attB53_SNCB-_attB53

    Plasmid
    #183615
    Purpose
    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Available Since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183615-plasmid-map-sequence-id-366916

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  12. EGFP-acVHH (Y34W)-PB

    Plasmid
    #171072
    Purpose
    A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34W
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171072-plasmid-map-sequence-id-338143

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  13. EGFP-acVHH (Y34F)-PB

    Plasmid
    #171071
    Purpose
    A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34F
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171071-plasmid-map-sequence-id-338141

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  14. EGFP–acVHH (Y104F)–PB

    Plasmid
    #169683
    Purpose
    A COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104F
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169683-plasmid-map-sequence-id-338137

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  19. pNeae2

    Plasmid
    #168300
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:
    Insert
    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    Tags
    6xHis, E-tag, and myc-tag
    Expression
    Bacterial
    Promoter
    LacI-Plac
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168300-plasmid-map-sequence-id-334450

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  21. nCaMK2rep

    Plasmid
    #239624
    Purpose
    CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Article
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Synthetic, Rat)
    Use
    Cre/Lox and Lentiviral
    Tags
    Flag and myc
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239624-plasmid-map-sequence-id-476245

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  22. nCaMK2rep-S603A

    Plasmid
    #239625
    Purpose
    Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Article
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Synthetic, Rat)
    Use
    Cre/Lox and Lentiviral
    Tags
    Flag and myc
    Expression
    Mammalian
    Mutation
    Changed serine 603 to Alanine
    Promoter
    hSyn
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239625-plasmid-map-sequence-id-476241

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  26. pAW212

    Plasmid
    #172196
    Purpose
    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites
    Depositor
    Chang Liu
    Article
    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038
    Insert
    p10B2-AT110
    Expression
    Yeast
    Promoter
    10B2 (P1 specific promoter)
    Available Since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172196-plasmid-map-sequence-id-338565

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  30. pMT[Hygro]_GBP-3xFlag-DEST

    Plasmid
    #157813
    Purpose
    A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection
    Depositor
    Zoltan Lipinszki
    Article
    Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:
    Insert
    GFP-binding protein
    Use
    For drosophila cells
    Tags
    3xFlag
    Expression
    Insect
    Promoter
    metallothionein
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    157813-plasmid-map-sequence-id-311081

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Showing: 841 - 870 of 886 results