We narrowed to 1,593 results for: PTS
-
Plasmid#127749PurposeLentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only
-
pSTAP-seq_human_spike-in
Plasmid#125152PurposeEmpty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cellsDepositorTypeEmpty backboneUseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_007
Plasmid#123760PurposeEncodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK systemDepositorInsertPTS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mfR
Plasmid#60991PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKate2.DepositorInsertmKate2
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mG
Plasmid#60987PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EGFPDepositorInsertEGFP
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mR
Plasmid#60990PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomatoDepositorInsertdTomato
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_007
Plasmid#123821PurposeEncodes Peroxisome Targeting Sequence as a Type 4a part to be used in the MTK systemDepositorInsertPTS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hfR
Plasmid#60998PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear mKate2.DepositorInsertmKate2
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-tWnt5a
Plasmid#169372PurposeExpresses a part of Chinese soft-shell turtle Wnt5a for in situ hybridization probe.DepositorInsertWnt5a
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-tWnt7b
Plasmid#169371PurposeExpresses a part of Chinese soft-shell turtle Wnt7b for in situ hybridization probe.DepositorInsertWnt7b
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mB
Plasmid#60985PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.DepositorInsertEBFP2
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mO
Plasmid#60989PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKODepositorInsertmKO
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mY
Plasmid#60988PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound citrineDepositorInsertcitrine
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mC
Plasmid#60986PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound ceruleanDepositorInsertCerulean
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Peroxisomes-2
Plasmid#54664PurposeLocalization: Peroxisomes, Excitation: 462, Emission: 492DepositorTypeEmpty backboneUseTagsPeroximal Targeting Signal 1 (PTS1) and mTFP1ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJJB1357
Plasmid#218595PurposeExpress Pex11 and the degron assay: Degradation tag-YFP-ePTS1DepositorInsertPex11
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1439
Plasmid#218589PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniolDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationErg20(F96W, N127W)PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS1461
Plasmid#218587PurposeExpress peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPPDepositorInsertErg12
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S1.46G>U
Plasmid#204160PurposepRegev_S1 with single mutation that disrupts secondary structure.DepositorInsertS1 exon with 46G>U substitution in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation46G>U substitutionPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S2.39U>G
Plasmid#204163PurposepRegev_S2 with single mutation that disrupts secondary structure.DepositorInsertExon S2 with 39U>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.51A>C
Plasmid#204172PurposepRegev_S4 with single mutation that disrupts secondary structure.DepositorInsertExon S4 with 51A>C mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation51A>C mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.39U>G
Plasmid#204171PurposepRegev_S4 with single mutation that disrupts secondary structure.DepositorInsertExon S4 with 39U>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S5.49C>A
Plasmid#204175PurposepRegev_S5 with single mutation that disrupts secondary structure.DepositorInsertExon S5 with 49C>A mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK127
Plasmid#200139PurposeType 4a part for Modular Cloning in YeastDepositorInsertFox2-PTS1 (870-900)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRenilla
Plasmid#118059PurposeGBPart - Renilla Luciferase CDSDepositorInsertRenilla
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIBR003
Plasmid#177666PurposeSIBR Intron 3 (Int3) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Alpha1
Plasmid#118044PurposeGoldenBraid2.0 Alpha 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP PR-D25A
Plasmid#101334PurposepBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activityDepositorInsertNL4-3 envFS
UseLentiviralTagsExpressionMutationProtease D25APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pELuc
Plasmid#118056PurposeGBPart - ELuc Luciferase CDSDepositorInsertELuc
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Omega 1
Plasmid#118046PurposeGoldenBraid2.0 Omega 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiniP
Plasmid#118049PurposeGBPart - Minimal Promoter for expression in mammalian cellsDepositorInsertminimal TATA-box promoter with low basal activity
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP IN-D116A
Plasmid#101333PurposepBS NL4-3 envFS eGFP with mutation that disrupts IN catalytic activityDepositorInsertNL4-3 envFS
UseLentiviralTagsExpressionMutationIntegrase D116APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP RT-D185K/D186L
Plasmid#101332PurposepBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activityDepositorInsertNL4-3 envFS
UseLentiviralTagsExpressionMutationreverse transcriptase D185K/D186LPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Omega 2
Plasmid#118047PurposeGoldenBraid2.0 Omega 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Alpha2
Plasmid#118045PurposeGoldenBraid2.0 Alpha 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRPa-iSL(MCS1+2)
Plasmid#69244PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Dest_R1-R3
Plasmid#140878Purpose166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.DepositorInsertccdb/CAM
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIBR002
Plasmid#177665PurposeSIBR Intron 2 (Int2) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIBR001
Plasmid#177628PurposeSIBR Intron 1 (Int1) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p alpha-actinin:GAL4/UAS:TdTomato
Plasmid#161712PurposeExpress TdTomato through amplification of GAL4/UAS systemDepositorInsertTdTomato, GAL4
UseXenopus laevis expressionTagsTdTomatoExpressionMammalianMutationPromoteractin-GAL4, UASAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ctrl
Plasmid#86380PurposeVector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorTypeEmpty backboneUseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJJB1559
Plasmid#218590PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationErg20(F96W, N127W), Erg20-Erg20 synthetic homodim…PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly_spike-in
Plasmid#125151PurposeVector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cellsDepositorInsertD. pseudoobscura peak #47 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPa-iSL(MCS3+4)
Plasmid#69246PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB1F+6
Plasmid#190835PurposeFor mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLemir9-124
Plasmid#31779DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-24xMS2
Plasmid#212629PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTight-9-124
Plasmid#31874DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only