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pSLS.405

Plasmid

#187203

Purpose

Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA (wildtype)
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTre2tet)::Fbxl21

Plasmid

#51890

Purpose

expression of Fbxl21

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl21 (Fbxl21 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV tet

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pTrcHisB mLCAD (29-430 aa)

Plasmid

#24490

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-28 deleted encodes active 29-430

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140)

Plasmid

#240582

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTG-mTDGa.1

Plasmid

#81049

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HLH Bmal Pas 1-465-VenC

Plasmid

#47364

Purpose

Bmal fragment cloned with Venus tag and used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC I317D

Plasmid

#47367

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, I317D

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L74E

Plasmid

#47355

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCK083 desmaMCS:minpromEGFP,exorh:mCherry

Plasmid

#200016

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

Plasmid

#121831

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-dCas9(3.7)/LSD1 (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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attB‐mCh‐Poly(A)-PuroR

Plasmid

#65529

Purpose

PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

Plasmid

#234952

Purpose

Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p11d-RPA70/14 (MSW#134)

Plasmid

#208076

Purpose

Expression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tag

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA3 (RPA1, RPA3 Human)

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTCO2

Plasmid

#149428

Purpose

cloning vector with regulatory regions of the murine Tdg gene

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

murine Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBHDuet40

Plasmid

#124358

Purpose

IPTG-inducible vector encoding the N-terminal gp41-1 split fragment with N-terminally His-tagged GB1

Depositor

Hideo Iwai

Article

Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

Insert

gp41-1 N-intein

Tags

His6-GB1

Expression

Bacterial

Promoter

T7

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHBSTC146

Plasmid

#124357

Purpose

Plasmid expressing inactive N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

Depositor

Hideo Iwai

Article

Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

Insert

gp41-1 N-intein (C1G) and C-intein (bicistronic)

Tags

CcdA-C (SFAD)

Expression

Bacterial

Mutation

C1G

Promoter

STC

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

Plasmid

#159575

Purpose

Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)

Tags

His6-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCK082 desmaMCS:minprommCherry,exorh:mCerulean

Plasmid

#200015

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

Plasmid

#121824

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/LSD1 (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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