We narrowed to 949 results for: GGCT;

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pLKO.1 puro shSLC2A1-2

Plasmid

#193703

Purpose

Constitutive lentiviral expression of SLC2A1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLCHKO_HPRT1_exon2_1_Lb

Plasmid

#155051

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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sg_Brd9_i5-ipUSEPR-TR657

Plasmid

#228922

Purpose

Knockdown of Brd9 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Brd9_i5 (Brd9 Mouse)

Use

Lentiviral

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

Plasmid

#163169

Purpose

Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

Non-targeting

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLCHKO_HPRT1_exon3_1_Lb

Plasmid

#155053

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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L2_lacZgRNA-Cas9-CsA

Plasmid

#136138

Purpose

Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA1-gRNA_A

Plasmid

#74374

Purpose

gRNA_A to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145694)

Plasmid

#80191

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.1

Plasmid

#91895

Purpose

sgNf1.1 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.1

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (CTNNB1)

Plasmid

#180193

Purpose

AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MAVS-ts3

Plasmid

#174276

Purpose

MAVS knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MAVS shRNA (MAVS Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin2a

Plasmid

#124850

Purpose

Mutagenesis of Grin2a

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2a (Grin2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDL691

Plasmid

#231166

Purpose

T-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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sgRNA1_ASCL1

Plasmid

#64129

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL1 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-shSmad2

Plasmid

#37051

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad2 (SMAD2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
PKN3 gRNA (BRDN0001146792)

Plasmid

#77948

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN3 (PKN3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRCN0000072225

Plasmid

#78160

Purpose

shLacZ control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

LacZ

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
gh136

Plasmid

#106806

Purpose

expression of gRNA targeting EXT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

EXT2 (EXT2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v2 puro

Plasmid

#87791

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147780)

Plasmid

#80226

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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