We narrowed to 589 results for: pcDNA3 expression vector

1. pCMV-ACBEmax

   Plasmid

   #139106

   Purpose

   Mammalian ACBEmax expression vector (pSI-632)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. S113

   Plasmid

   #174551

   Purpose

   Constitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)

   Depositor

   Bruno Correia

   Article

   Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

   Insert

   mdm2 and designed protein LD6 (MDM2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

   Plasmid

   #166804

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S1213A

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FL-BCL2L1

   Plasmid

   #78860

   Purpose

   DULIP positive control (prey) for assay establishment

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   BCL2 like 1 (BCL2L1 Human)
   

   Use

   Expression vector

   Tags

   Firefly luciferase, V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LUC2 (L/Sec/deltaP)

   Plasmid

   #112145

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and d…

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGlomyc-Klf4

   Plasmid

   #172870

   Purpose

   Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

   Depositor

   Renee van Amerongen

   Article

   Mourao et al bioRxiv 2021.06.22.449461

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LUC45 (L/Sec/mutP)

   Plasmid

   #112148

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and SE…

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PA-RL-BAD

   Plasmid

   #78859

   Purpose

   DULIP positive control (bait) for assay establishment

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   BCL2 associated agonist of cell death (BAD Human)
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-sgE1TSS-short-3'box

   Plasmid

   #92165

   Purpose

   sgRNA expression vector for Evx1as RNA tethering assay

   Depositor

   Xiaohua Shen

   Article

   Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

   Insert

   Evx1as short isoform
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 KBTBD4 WT

    Plasmid

    #184627

    Purpose

    Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    no mutation

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_OHu19986C_myc tag

    Plasmid

    #183174

    Purpose

    used to make AAV vectors for hsTRPA1 tagged with myc

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Insert

    hsTRPA1 (TRPA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA 3.1 KBTBD4 R313PRR

    Plasmid

    #184629

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R313PRR expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R313PRR (KBTBD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R313PRR

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 KBTBD4 P311PP

    Plasmid

    #184628

    Purpose

    Mammalian expression vector with N-terminal 3xFlag for KBTBD4 P311PP expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 P311PP (KBTBD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P311PP

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA 3.1 KBTBD4 R312RG

    Plasmid

    #184630

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R312RG

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

    Plasmid

    #166809

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1322R

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R

    Plasmid

    #166810

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    LL1193EE, P1214R

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE

    Plasmid

    #166805

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    LL193EE

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E

    Plasmid

    #166808

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K1277E

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G

    Plasmid

    #166807

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H1276G

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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