We narrowed to 5,093 results for: puc

1. pUC18/promSau/Lysostaphin

   Plasmid

   #125766

   Purpose

   Production of staphylolytic protein lysostaphin from a constitutive promoter

   Depositor

   Benedykt Wladyka

   Article

   Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

   Insert

   lysostaphin
   

   Expression

   Bacterial

   Mutation

   sequence corresponding to the mature form protein

   Promoter

   pemIK constitutive promoter

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

   Plasmid

   #155306

   Purpose

   Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA BFP
   

   Expression

   Mammalian

   Promoter

   hU6, EF1a

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57 mini-ZsGreen

   Plasmid

   #222648

   Purpose

   Vector for cloning gene of interest for in vitro transcription

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   ZsGreen
   

   Use

   In vitro transcription

   Mutation

   mutated stop codon

   Promoter

   T7

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-Mu-switch-R-loop

   Plasmid

   #134899

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   mouse IgH switch repeat region
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T

   Plasmid

   #64957

   Purpose

   mini-Tn7 base vector with transcriptional terminator and oriT

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pUC57-Amp_CRISPIE donor B2

   Plasmid

   #172843

   Purpose

   CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mRuby3
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC118-FLIP-Puro

   Plasmid

   #84538

   Purpose

   a mock vector for generating FLIP targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pUC57-HBB-nLuc

   Plasmid

   #175432

   Purpose

   Used to generate IVT RNA for translation extracts

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   HBB-nLuc
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-UAS1B8-Leum

   Plasmid

   #44316

   Depositor

   Hal Alper

   Article

   Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

   Insert

   UAS1B8-Leum promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   UAS1B8-Leum

   Available Since

   April 18, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pUCmini-iCAP-PHP.V2

    Plasmid

    #127848

    Purpose

    non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.V2 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUCmini-iCAP-PHP.B4

    Plasmid

    #127849

    Purpose

    non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.B4 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-U6-terminator (SL695)

    Plasmid

    #133961

    Purpose

    pUC19 plasmid with U6 promoter followed by a poly-T termination signal

    Depositor

    Keith Joung

    Insert

    U6 promoter
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-white[coffee]

    Plasmid

    #84006

    Purpose

    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

    Depositor

    Phillip Zamore

    Article

    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

    Insert

    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    

    Use

    Unspecified

    Mutation

    A GC-to-AA mutation that creates a G589E missense…

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. His Puc (aa 1-393)

    Plasmid

    #52295

    Purpose

    Bacterial expression of His tagged Drosophila Puc fragment

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    Puc (puc Fly)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pUC-T7-J1~398-Luc

    Plasmid

    #208639

    Purpose

    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19

    Depositor

    Quanfu Ma

    Insert

    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    

    Use

    Luciferase; In vitro transcription template

    Tags

    Firefly Luciferase (F-Luc and T7 promoter

    Mutation

    nt 1~398, in frame with donstream F-Luc

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCBB-pT7-eGFP

    Plasmid

    #32552

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Promoter

    pT7

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. IMBB2.4-pUC57-mini

    Plasmid

    #88845

    Purpose

    plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2017 Mar 1;62(3):99-106.

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCBB-ctH6-eGFP

    Plasmid

    #32562

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    6 His tag with Thrombin site

    Promoter

    Consitutive lac

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCmini-UGCG-tdTomato

    Plasmid

    #225879

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    tdTomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-YAP-mEGFP

    Plasmid

    #166472

    Purpose

    HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)
    

    Expression

    Mammalian

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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