We narrowed to 3,452 results for: ttl

1. pDS2

   Plasmid

   #70135

   Purpose

   CEN/ARS yeast shuttle vector with DS2 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   DS2
   

   Use

   Synthetic Biology

   Promoter

   AgTEF1

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. wtTDP43tdTOMATOHA

   Plasmid

   #28205

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Tags

   HA tag and tdtomato

   Expression

   Mammalian

   Available Since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. mcherry-Myo 9b

   Plasmid

   #134918

   Purpose

   Expression of rat Myo 9b (Myr 5) in mammalian cells.

   Depositor

   Martin Bähler

   Article

   Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

   Insert

   Myo 9b (rat) with 3' UTR (Myo9b Rat)
   

   Tags

   mcherry

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. hCas9-VPR

   Plasmid

   #68497

   Purpose

   nuclease competent SP-Cas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   SP-Cas9-VPR
   

   Tags

   VPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBintT

   Plasmid

   #48134

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWM_12x601_25bpLinker

   Plasmid

   #157788

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_25bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWM_12x601_15bpLinker

   Plasmid

   #157786

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_15bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWM_12x601_30bpLinker

   Plasmid

   #157789

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_30bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWM_12x601_20bpLinker

   Plasmid

   #157787

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_20bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWM_12x601_45bpLinker

    Plasmid

    #157791

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_45bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHBintE

    Plasmid

    #48136

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWM_12x601_35bpLinker

    Plasmid

    #157790

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_35bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR221-Renilla

    Plasmid

    #79496

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Renilla Luciferase
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKEK3230

    Plasmid

    #234102

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    FuGFP
    

    Expression

    Bacterial

    Promoter

    pJ23100-BsaI

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOZITX-S1

    Plasmid

    #184925

    Purpose

    Expresses the catalytic S1 subunit of OZITX (EcPltAB)

    Depositor

    Jonathan Javitch

    Article

    Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

    Insert

    codon optimized S1 subunit of OZITX
    

    Expression

    Mammalian

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRPF215

    Plasmid

    #106377

    Purpose

    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

    Insert

    Ptet-himar1-Ter(slpA)-transposon
    

    Use

    E. coli - c. difficile shuttle vector

    Mutation

    Codon optimised for C. difficile

    Promoter

    Ptet

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR221-mCherry

    Plasmid

    #79509

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    mCherry RFP
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSC168

    Plasmid

    #216350

    Purpose

    xhNup35-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup35-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDG04163

    Plasmid

    #216373

    Purpose

    xhNup214-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup214-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-scSUMO

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA_tdtomato_reporter_1_SA-2x

    Plasmid

    #79369

    Purpose

    Assays activity of transcriptional activators fused to SA Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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