59,541 results

1. pmCherry_a_tubulin_IRES_puro2

   Plasmid

   #21043

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42

   Plasmid

   #111746

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q42 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-2xFLAG-2xSTREP-CCND2

   Plasmid

   #172629

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. JB84

   Plasmid

   #128908

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab9AQL-BirA*-HA-MAO (RAB9A Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304-VHL-IRES-GFP

   Plasmid

   #187641

   Purpose

   VHL Expression vector with GFP

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   VHL (VHL Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIGAhRC

   Plasmid

   #112510

   Purpose

   human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

   Depositor

   Charles Miller

   Article

   Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

   Insert

   HIF1B, AhR (ARNT, AHR Human, Budding Yeast)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Gal1,10

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACEBac1-GCP2

   Plasmid

   #178068

   Purpose

   Insect cell expression of GCP2

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   GCP2 (TUBGCP2 Human)
   

   Expression

   Insect

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-myc-ERK2-L4A-MEK1_fusion

   Plasmid

   #39197

   Depositor

   Melanie Cobb

   Article

   Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

   Insert

   ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   L4A = L33A, L37A, L40A, L42A

   Promoter

   CMV

   Available Since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196078

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5M-ADAM10

    Plasmid

    #31717

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    ADAM10 (ADAM10 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. Integrin alpha L - mCFP

    Plasmid

    #8637

    Depositor

    Timothy Springer

    Article

    Kim et al Science 2003 Sep 19;301(5640):1720-5.

    Insert

    Integrin, alpha L (ITGAL Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. CDC40

    Plasmid

    #155444

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CDC40 (CDC40 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-PTEN

    Plasmid

    #116780

    Purpose

    Lentiviral expression of PTEN

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LCV2 V-1

    Plasmid

    #84804

    Purpose

    Cas9 with sgRNA targeting Exon 1 of VEGF-A

    Depositor

    Glenn Yiu

    Article

    Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

    Insert

    gRNA targeting VEGF-A (VEGFA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. XX01CAPN2A-c001

    Plasmid

    #175492

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    CAPN2 / CAPNS1 (CAPN2 Human)
    

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIneo-V5-SBP-MBP-HsStaufen1-T2_V

    Plasmid

    #147743

    Purpose

    Mammalian Expression of HsStaufen1-T2

    Depositor

    Elisa Izaurralde

    Insert

    HsStaufen1-T2 (STAU1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2998

    Plasmid

    #144474

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSOX11.1.0-gDNA

    Plasmid

    #176378

    Purpose

    Expresses spCas9 and gRNA sequence targeting SOX11

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX11 gRNA
    

    Use

    CRISPR

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR TERRA

    Plasmid

    #111383

    Purpose

    TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    TERRA
    

    Use

    Gateway entry cloning

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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