We narrowed to 30,195 results for: REP

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pGL2_172

Plasmid

#199104

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI plus NGS barcode 20, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCm No carrier NikJC199U

Plasmid

#174361

Purpose

Expression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GXB

Plasmid

#160926

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEE22

Plasmid

#161787

Purpose

Hi-SC2 reporter delivery plasmid

Depositor

François-Xavier Barre

Article

Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

Insert

Hi-SC2 reporter

Use

Conjugative plasmid to deliver hi-sc2 reporter

Mutation

Himar site included MmeI site, tandem loxP sites,…

Promoter

n/a

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - L39V)

Plasmid

#136607

Purpose

Expresses DANCER-3 mutant (reversion L39V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (L39V reversion)

Tags

5x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - DANCER-2

Plasmid

#136386

Purpose

Expresses DANCER-2 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-2

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-S

Plasmid

#136388

Purpose

Expresses NERD-S static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-S

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-C

Plasmid

#136389

Purpose

Expresses NERD-C static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-C

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-F30L-V39I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I54V)

Plasmid

#136608

Purpose

Expresses DANCER-3 mutant (reversion I54V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I54V reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F34A)

Plasmid

#136606

Purpose

Expresses DANCER-3 mutant (reversion F34A)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F34A reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I7L)

Plasmid

#136605

Purpose

Expresses DANCER-3 mutant (reversion I7L)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I7L reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F3Y)

Plasmid

#136604

Purpose

Expresses DANCER-3 mutant (reversion F3Y)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F3Y reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHTR235syn

Plasmid

#133464

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR235

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHTR400syn

Plasmid

#133465

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR400

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHTR625syn

Plasmid

#133466

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR625

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHTR060syn

Plasmid

#133462

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR060

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHTR120syn

Plasmid

#133463

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR120

Expression

Bacterial

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pNOC-stacked-GeNL-Ca

Plasmid

#101003

Purpose

Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis expression

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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LPhP-pHG165

Plasmid

#90047

Purpose

Constitutive expression of a chimeric LacI:PurR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LPhP

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAN-PA5-RFP

Plasmid

#62251

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA5

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSKB2-TRexR90D

Plasmid

#31350

Depositor

Clara Kielkopf

Article

McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

Insert

Thermus aquaticus Redox Sensing Repressor (R90D)

Tags

His

Expression

Bacterial

Mutation

changed Arginine 90 to Aspartic Acid

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pSL5 SMR1713

Plasmid

#28028

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutL (mutL E. coli)

Expression

Bacterial

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pJF6

Plasmid

#21678

Depositor

James Haber

Article

Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Mutation

HO digestion site in one of the LacZ genes

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pJC25R1+8stop

Plasmid

#17724

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+8 (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pJC25R1+8H1Qstop

Plasmid

#17725

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+4 (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pSIRV-NFAT-eGFP

Plasmid

#118031

Purpose

Fluorescent reporter for NFAT activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eGFP

Use

Retroviral

Promoter

minimal promoter with NFAT binding site

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSIRV-AP-1-mCherry

Plasmid

#118095

Purpose

Fluorescent reporter for AP-1 activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

mCherry

Use

Retroviral

Promoter

minimal promoter with AP-1 responsive element

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Promoter

minimal promoter with NF-kB responsive element

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-mCD8

Plasmid

#162747

Purpose

NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMT525

Plasmid

#131392

Purpose

L1RP retrotransposition reporter

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP GFP-AI

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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