We narrowed to 18,064 results for: Gateway

1. pCPP3417

   Plasmid

   #128724

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopY1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ23119-sgRNA

   Plasmid

   #113654

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter J23119 and sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ149

   Plasmid

   #98407

   Purpose

   Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Inserts

   upstream homologous region to delete his3 in genome by recombination (alg2 Fission Yeast)
   downstream homologous region to delete his3 in genome by recombination (cog8 Fission Yeast)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE- TRE-DEST-NBioTAP

   Plasmid

   #53568

   Purpose

   Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

   Depositor

   Mitzi Kuroda

   Article

   Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC5

   Plasmid

   #104779

   Purpose

   pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAV150

   Plasmid

   #79764

   Purpose

   binary DEST vector for plant expression

   Depositor

   Nico Dissmeyer, Jean Greenberg

   Article

   Vinatzer et al Mol Microbiol. 2006 Oct;62(1):26-44. Epu

   Type

   Empty backbone

   Use

   Tetr

   Tags

   Expression

   Plant

   Mutation

   Promoter

   DEX

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC_C_EPHB4

   Plasmid

   #187768

   Purpose

   MAC-tagged gene expression of human EPHB4

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   EPHB4 (EPHB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. OLIG2_pCSdest

   Plasmid

   #53858

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   OLIG2 (OLIG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pFPNL1

   Plasmid

   #61651

   Purpose

   Gateway vector for generating nucleus-targeted fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   monomeric red fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-CMVSP6-NR2F2-SV40-PURO

    Plasmid

    #138361

    Purpose

    Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selection

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Human NR2F2, with puromycin driven by SV40 for selection
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCellFree_G03 IL1B

    Plasmid

    #67066

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IL1B (IL1B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCellFree_G03 CALR

    Plasmid

    #67086

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CALR (CALR Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pSws1-nfsB-CFP

    Plasmid

    #86375

    Purpose

    for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    nitroreductase (nfsB bacteria)
    

    Use

    Zebrafish

    Tags

    ECFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZA8

    Plasmid

    #158486

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    L17E, D481V, STOP codon

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ262m3

    Plasmid

    #149556

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, R221K, N394K, Protospacer interacting domai…

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAC_N_SOX10

    Plasmid

    #167791

    Purpose

    Expresses SOX10 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEn-C1.1

    Plasmid

    #61479

    Purpose

    Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

    Depositor

    Holger Puchta

    Article

    Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PRDM15_pCSdest

    Plasmid

    #53907

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PRDM15 (PRDM15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pGem-nHyPer2:sHyper2

    Plasmid

    #84737

    Purpose

    Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Use

    Tags

    NLS-SV40

    Expression

    Plant

    Mutation

    Promoter

    pFMV/p35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAC_N_NKX25

    Plasmid

    #167785

    Purpose

    Expresses NKX25 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    NKX25 (NKX2-5 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST40-Arl13b11

    Plasmid

    #40873

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    no stop codon

    Promoter

    CMV

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pUB-cSypHer

    Plasmid

    #84733

    Purpose

    Expresses SypHer in plant cells-biosensor for pH

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    SypHer
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]

    Plasmid

    #159900

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mcherry | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pmlc-1

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCLX-M38s/TOP-dGFP

    Plasmid

    #114295

    Purpose

    Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    dGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    destabilized GFP

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDSHA-SARA-Myc

    Plasmid

    #67368

    Purpose

    Mammalian expression of hSara with C-term Myc tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pDONR221-kcnk3a

    Plasmid

    #208985

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk3a (kcnk3a Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGATOR-DTA

    Plasmid

    #64103

    Purpose

    Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

    Depositor

    Bill Skarnes

    Article

    Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

    Insert

    rTTA (Gt(ROSA)26Sor Synthetic)
    

    Use

    Mouse Targeting; Tet inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ROSA26 endogenous

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAC-SARS-CoV-2 Orf9b

    Plasmid

    #158378

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 Orf9b
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. PFKL_pCSdest

    Plasmid

    #53901

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PFKL (PFKL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

30. MAC_N_TBR1

    Plasmid

    #167803

    Purpose

    Expresses TBR1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    TBR1 (TBR1 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MAC_N_PSA1

    Plasmid

    #111690

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PSA1 (PSMA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MRAP_pCSdest

    Plasmid

    #53833

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    MRAP (MRAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

33. PB-Sox17

    Plasmid

    #60669

    Purpose

    PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Sox17 (Sox17 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. T5-sgRNA

    Plasmid

    #113655

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter T5 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAWH

    Plasmid

    #133894

    Purpose

    Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJW4.02

    Plasmid

    #131372

    Purpose

    expression of MBP:PGL-1 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    pgl-1 (pgl-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCellFree_G06_ZIKV NS5

    Plasmid

    #145358

    Purpose

    Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS5
    

    Use

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-Zyxin-Full Length (aa1-564)-EGFP

    Plasmid

    #187692

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR4-V5-2-Ubc9 (w345-2)

    Plasmid

    #17407

    Purpose

    Gateway entry vector for N-term V5 tag, frame B and Ubc9 C-term

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Ubc9 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MAC_N_SOX15

    Plasmid

    #167792

    Purpose

    Expresses SOX15 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    SOX15 (SOX15 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge