We narrowed to 25,008 results for: CHI
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Plasmid#172617PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(P284L) and a puromycin resistance marker in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only
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pMSCV-FLAG-2xSTREP-CCND2(P281L)-Puro
Plasmid#172624PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(P281L) and a puromycin resistance marker in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
px458-AMBRA1 #3
Plasmid#172607PurposeExpresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFPDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
px458-AMBRA1 (optimized for N-terminal knock-in)
Plasmid#172608PurposeExpresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.DepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA
Plasmid#136657PurposeExpresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNADepositorInsertsUseCRISPR and Synthetic BiologyTags2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9ExpressionMammalianPromoterCAG and U6Available SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA
Plasmid#136654PurposeExpresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNADepositorInsertsUseCRISPR and Synthetic BiologyTags2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9ExpressionMammalianPromoterCAG and U6Available SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3
Plasmid#124435PurposeChimeric ETS domain: H3 of Ets-1 in PU.1 scaffoldDepositorTags6xHis TagExpressionBacterialMutationResidues 225 to 239 replaced with corresponding s…Available SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Wing
Plasmid#124552PurposeChimeric ETS domain: Wing of Ets-1 in PU.1 scaffoldDepositorTags6xHis tagExpressionBacterialMutationThe 5 residues making up the "wing" in …Available SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Loop
Plasmid#124551PurposeChimeric ETS domain: Loop of Ets-1 in PU.1 scaffoldDepositorTags6xHis tagExpressionBacterialMutationResidues 216 to 223 replaced with corresponding s…Available SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PU.1-ETS N236H
Plasmid#124616PurposeChimeric ETS domain: N236HDepositorExpressionMammalianMutationResidues 236 altered from asparagine to a histidi…Available SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PU.1-ETS N236Q
Plasmid#124615PurposeChimeric ETS domain: N236QDepositorExpressionMammalianMutationResidues 236 altered from asparagine to a glutami…Available SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PU.1-ETS N236Y
Plasmid#124614PurposeChimeric ETS domain: N236YDepositorExpressionMammalianMutationResidues 236 altered from asparagine to a TyrosineAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3/S3
Plasmid#124627PurposeChimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffoldDepositorTags6xHis tagExpressionBacterialMutationResidues 237 to 241 replaced with corresponding s…Available SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-S3
Plasmid#124550PurposeChimeric ETS domain: S3 of Ets-1 in PU.1 scaffoldDepositorTags6xHis tagExpressionBacterialMutationResidues 242 to 246 replaced with corresponding s…Available SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS-H2
Plasmid#123358PurposeChimeric ETS domain: H2 of Ets-1 in PU.1 scaffoldDepositorTags6xHis tagExpressionBacterialMutationResidues 207 to 215 replaced with corresponding s…Available SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant
Plasmid#72924PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseGateway CloningTags3XAU1 and 3XFLAGExpressionBacterialMutationSerine#37 changed to Alanine;Silent mutations mad…PromoterTRE tightAvailable SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant
Plasmid#72617PurposeExpresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationSerine#37 changed to Alanine;Silent mutations mad…PromoterTRE tightAvailable SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-CFP
Plasmid#50482Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsCFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the CFP open…PromoterCMVAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)
Plasmid#174516PurposeRecoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.DepositorInsertsPylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon
ExpressionBacterialMutationCUA anticodon, M.alvus Pyl tRNA with CGA anticodo…PromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only