We narrowed to 41,541 results for: ids

1. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

   Plasmid

   #20297

   Purpose

   Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   channelrhodopsin-2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   humanized ChR2 gene with histidine 134 changed to…

   Promoter

   EF1alpha

   Available Since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

   Plasmid

   #85225

   Purpose

   Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

   Depositor

   John Naughton

   Inserts

   Optimized G Protein
   Enhanced GFP
   TVA (CD320 Chicken)
   

   Use

   AAV and Cre/Lox

   Promoter

   hSyn

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRTi

   Plasmid

   #214072

   Purpose

   Luminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LRTX_ctrl

   Plasmid

   #214073

   Purpose

   Luminescent reporter system, NMD negative control. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Remnant of linker sequence present at the end of …

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Gal4ff.zf1

   Plasmid

   #61392

   Purpose

   mRNA synthesis of Gal4ff codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Gal4ff.zf1
   

   Use

   Zebrafish expression

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJKR-O-mphR

   Plasmid

   #62570

   Purpose

   Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. LRT_RTctrl

   Plasmid

   #214074

   Purpose

   Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LRT

   Plasmid

   #214071

   Purpose

   Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWPI-IRES-Bla-Ak-ACE2-TMPRSS2

   Plasmid

   #154983

   Purpose

   Lentiviral vector expressing Human ACE2 and TMPRSS2

   Depositor

   Sonja Best

   Insert

   ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LiRT

    Plasmid

    #214075

    Purpose

    Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LiRT_RTctrl

    Plasmid

    #214078

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LiRTi

    Plasmid

    #214076

    Purpose

    Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LiRTX_ctrl

    Plasmid

    #214077

    Purpose

    Luminescent reporter system, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRESN2_GFP_YTA_Champ1_328-354

    Plasmid

    #105639

    Purpose

    Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP

    Depositor

    Thanos Halazonetis

    Insert

    Champ1 (CHAMP1 Human)
    

    Tags

    GFP YTA

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid

    #105714

    Purpose

    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

    Depositor

    Rylan Larsen

    Has Service

    AAV8

    Insert

    GCaMP6s
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEmerald-ERBB2-N-18

    Plasmid

    #62755

    Purpose

    Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    ERBB2 (ERBB2 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pelBHisTEV

    Plasmid

    #157741

    Purpose

    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Tags

    PelB signal sequence + His10-tag + TEV protease c…

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p159 pPr-luc

    Plasmid

    #8392

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    probasin promoter (Pbsn Rat)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

19. RP4-6

    Plasmid

    #80385

    Purpose

    Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Spectinomycin/Streptomycin
    

    Use

    Helper bhr plasmid for mobilizing other plasmids …

    Tags

    none

    Promoter

    None

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pALS3-Ma-sfGFP-TAG150

    Plasmid

    #212121

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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