We narrowed to 10,433 results for: yeast

Showing: 8761 - 8790 of 10433 results

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW012

Plasmid

#83527

Purpose

Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBKWH

Plasmid

#133898

Purpose

Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ235: pRS426-His-MBP-GtFz1_Native

Plasmid

#205254

Purpose

S. cerevisiae expression vector for GtFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- PGK1prom-TIR1-FLAG

Plasmid

#189724

Purpose

Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Expression

Yeast

Promoter

PGK1 promoter

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-URA3-3'SEC13-GFP

Plasmid

#25445

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

Insert

Sec13-Green Fluorescent Protein

Expression

Yeast

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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MAP72_MS2

Plasmid

#220623

Purpose

Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Use

Shuttle vector

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pWS926

Plasmid

#123052

Purpose

LexA bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexA-NLS [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSDMA65

Plasmid

#128354

Purpose

Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.

Depositor

James Fraser

Article

Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

Type

Empty backbone

Use

CRISPR

Promoter

ACT1

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lifeact-Dronpa

Plasmid

#182330

Purpose

Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Lifeact-Dronpa

Tags

N-terminal 17 amino acids from budding yeast Abp1…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN0604 (pDEST, HygR)

Plasmid

#210483

Purpose

pDEST vector with HygR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0603 (pDEST, NatR)

Plasmid

#210482

Purpose

pDEST vector with NatR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCu-MCP-2yeGFP(405)

Plasmid

#121419

Purpose

Expressing MCP-2yeGFP

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

CUP1p-MCP-2yeGFP

Expression

Yeast

Promoter

CUP1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLexA::Ras

Plasmid

#11340

Depositor

Michael Wigler

Article

Chang et al Cell. 1994 Oct 7. 79(1):131-41.

Insert

Ras1 (ras1 Fission Yeast)

Tags

LexA DNA binding domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pBS-SKII-URA-GAL1-fCLIP-3xHA

Plasmid

#188936

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pYC34

Plasmid

#63915

Purpose

Recipient overexpressing vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pKar2-msGFP-HDEL

Plasmid

#117668

Purpose

Localisation of Endoplasmic Reticulum

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pKar2-msGFP-HDEL

Expression

Yeast

Mutation

Contains the Kar2 promoter and signal sequence fr…

Promoter

pKar2

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUpstream2Lox

Plasmid

#164573

Purpose

Integration of 2 LoxN sites in the genome of P. berghei

Depositor

Olivier Silvie

Inserts

Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Human, Synthetic)
bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)

Use

Cre/Lox

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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POM42-mCherry

Plasmid

#69856

Purpose

N-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Expression

Yeast

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL498

Plasmid

#169154

Purpose

Encodes OptoQ-INVRT4

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Expression

Yeast

Promoter

GPD

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pYC46

Plasmid

#63904

Purpose

Recipient integrative vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FLAG

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTR1-GPD-TRPC

Plasmid

#190103

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

Depositor

Perng-Kuang Chang

Article

Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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plIVMD156

Plasmid

#160934

Purpose

In vivo mRNA display Gateway Destination construct

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomSir1

Plasmid

#107447

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sir1 (sir1 Fission Yeast)

Tags

V5-His

Expression

Yeast

Mutation

H523R, A750T

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL724

Plasmid

#169151

Purpose

Encodes OptoQ-AMP5

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pKL1-222

Plasmid

#219409

Purpose

Nuclear expression of Synrophin PDZ clamp protein

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

pTEF1-NLS-(Syntrophin-PDZ)x4-tADH1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP-TAG

Plasmid

#158131

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Mutation

Introduced a stop codon in place of the tyrosine …

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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