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pD649-HAsp-SDK1(Trunc1)-Fc(DAPA)-AviTag-6xHis

Plasmid

#157051

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SDK1 (SDK1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP Mps1 DK

Plasmid

#63703

Purpose

Inducible expression of GFP-Mps1 DK fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

Mps1 (TTK Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 47-156, kinetochore binding domain

Promoter

tight TRE promoter

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FCGR3A-COMP5AP-AviTag-9xHis

Plasmid

#157167

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FCGR3A (FCGR3A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

Plasmid

#51249

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 980,1006 mutated to Ala

Promoter

EF1a

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only
Flag-M4-AR-W741C-S650A

Plasmid

#171252

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S650D

Plasmid

#171253

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

Plasmid

#51247

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907 mutated to Ala

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEMS1482

Plasmid

#29235

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple12 (AVP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Oct. 20, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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VDAC2_L (OZ523)

Plasmid

#27198

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting VDAC2 (vdac2 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
TTK gRNA (BRDN0001145152)

Plasmid

#75579

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTK gRNA (BRDN0001146504)

Plasmid

#75580

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTK gRNA (BRDN0001147779)

Plasmid

#75581

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TYRO3 gRNA (BRDN0001147880)

Plasmid

#76227

Purpose

3rd generation lentiviral gRNA plasmid targeting human TYRO3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TYRO3 (TYRO3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TYRO3 gRNA (BRDN0001146050)

Plasmid

#76228

Purpose

3rd generation lentiviral gRNA plasmid targeting human TYRO3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TYRO3 (TYRO3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TYRO3 gRNA (BRDN0001147403)

Plasmid

#76229

Purpose

3rd generation lentiviral gRNA plasmid targeting human TYRO3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TYRO3 (TYRO3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100/125/147/213-siResist

Plasmid

#49858

Purpose

Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, Methionine 147, an…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
NC12 pCDNA3.1 VENUS WTX delta ETGE

Plasmid

#36967

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX (delta ETGE motif) (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

lacks amino acids 288 –291

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
VDAC2_R (OZ524)

Plasmid

#27199

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting VDAC2 (vdac2 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
mCitrine-DNAJC5 L115R

Plasmid

#246735

Purpose

Mammalian expression of human DNAJC5 L115R with a mCitrine (YFP) tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCitrine

Expression

Mammalian

Mutation

L115R

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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mCerulean-DNAJC5

Plasmid

#246730

Purpose

Mammalian expression of human DNAJC5 wildtype with a mCerulean tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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