We narrowed to 24,925 results for: crispr
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Plasmid#140833PurposeEncoding the TRE promoter based eccDNA biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTRE-ECC Biosensor
UsePiggybac (pb) transposonExpressionMammalianPromoterinverseAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS
Plasmid#129532PurposeSelf-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4DepositorInsertscodon-optimized AcrIIA4
sgRNA_Rosa26
UseAAVTagsFLAG/NLSPromoterU6 and cytomegalovirus-enhancer chicken β-actin p…Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)
Plasmid#115142PurposeCAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized Mb3Cas12a
TagsNLS(nucleoplasmin) and 3xHAExpressionMammalianAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSDMA65
Plasmid#128354PurposeCodon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.DepositorTypeEmpty backboneUseCRISPRPromoterACT1Available sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ptreCas9-mKate2ps-T1gRNA
Plasmid#62715PurposeInducible; gRNA seq is ATGAGAATCAAGGCGGTCGADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9–mKate2ps
ExpressionMammalianAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
hgRNA-D21_pLKO-Hyg
Plasmid#100562PurposehgRNA-D21 in a lentiviral backboneDepositorInserthgRNA-D21
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hgRNA-C21_pLKO-Hyg
Plasmid#100561PurposehgRNA-C21 in a lentiviral backboneDepositorInserthgRNA-C21
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-dCas9-VPR-wpA-pPuroTK
Plasmid#214127PurposeGene expression by Piggybac transposase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-VPR
UseCRISPRExpressionMammalianMutationCodon-optimized for mammalian cellsAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-VP64-dCas9-VP64-ZsG
Plasmid#192668PurposeDox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1DepositorInsertVP64-dCas9-VP64, ZsGreen1
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterTRE3GAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY130 (pcDNA3.1-U6-huBsCpf1)
Plasmid#92306PurposeExpression of humanized BsCpf1 and crRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuBsCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY128 (pcDNA3.1-U6-huMb2Cpf1)
Plasmid#92304PurposeExpression of humanized Mb2Cpf1 and crRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuMb2Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY127 (pcDNA3.1-U6-huTsCpf1)
Plasmid#92303PurposeExpression of humanized TsCpf1 and crRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuTsCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102892PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXSE901B
Plasmid#91713PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistanceDepositorTypeEmpty backboneExpressionPlantAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC39
Plasmid#62333PurposesgRNA + 1x PP7 with mCherry for mammalian cellsDepositorInsertssgRNA + 1x PP7
mCherry
UseLentiviralExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1
Plasmid#118158PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV ORANGE Gria1-HaloTag KI
Plasmid#139655PurposeEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)DepositorInsertgRNA and HaloTag donor
UseAAVExpressionMammalianPromoterU6Available sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-OsPE2
Plasmid#141080PurposeGateway entry clone for CRISPR-Cas9(H840A) prime editingDepositorInsertzCas9(H840A)-OsM-MLV-RT
UseCRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rtTags3x FLAG, NLS and NLSExpressionPlantAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ133A2.0
Plasmid#99891PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCIPpay-BFP reporter
Plasmid#154096PurposeSelf-Cutting and Integrating Payload CRISPR backbone with BFP reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP2A-tagBFP
UseCRISPRExpressionMammalianAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only