We narrowed to 16,181 results for: nes
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Plasmid#199304PurposeFluorescence and bioluminescence expression in Gram-negative bacteriaDepositorInsertChloramphenicol acetyltransferase
ExpressionBacterialPromoterT7Available sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKMW122_SpG-Cas9_U6-entry
Plasmid#244819PurposeMammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassetteDepositorInsertSpG Cas9
UseCRISPRTags3xFLAG-SV40 NLS and Nucleoplasmin NLSExpressionMammalianMutationD1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCAG, U6Available sinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS
Plasmid#162770PurposeProtein expression in CHO cellsDepositorTypeEmpty backboneExpressionMammalianPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955
Plasmid#134613PurposeMammalian expression of rat KIF5C 378-955DepositorInsertKIF5C (Kif5c Rat)
TagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GCaMP7a
Plasmid#184817PurposeUsed to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).DepositorInsertQUAS:GCaMP7a-pA
UseZebrafish tol2 transgenesisAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
EC67908
Plasmid#211795PurposeOver expresses Cas12a and GR-GRF-GIF. Must receive guide cassettesDepositorInsertHptII, Cas12a, GR-GRF-GIF
ExpressionPlantAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
EC67842
Plasmid#209516PurposeCloning in complete guide expression cassettes, with Cas12a (D156R)DepositorTypeEmpty backboneUseCRISPRAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
791 p3E-2A-pCS2MCSpA v2
Plasmid#195973PurposeGateway p3E 2A fluorophoreDepositorInsert2A-multiple cloning site
UseOtherAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-miRFP670nano-PGK-Blast
Plasmid#196722PurposeTet-inducible expression of Cas9-T2A-miRFP670nanoDepositorInsertCas9-T2A-miRFP670nano
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD405
Plasmid#163110PurposeExpression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS_mScarlet-I_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
P526
Plasmid#139740PurposeIndividual component of Caffeic Acid biosynthesis pathway, hpaCDepositorInserthpaC
ExpressionPlantPromoter35SAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pALPS eGFP
Plasmid#101323PurposeEncodes eGFPDepositorInserteGFP
UseLentiviralAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pATP416-ptetO7-pphlO6-crtYBI
Plasmid#165976PurposeExpresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationC66A, C1359T and C1281A, G1698APromoterSynthetic promoter (pphlO6), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CLASP2 512-650 9xS/A
Plasmid#24410DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLASP2 (512-650) 9xS/A (CLASP2 Human)
TagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant that c…Available sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(AtUBI10)
Plasmid#202016PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
TagsGB1 and sfGFPExpressionPlantPromoterAtUBI10Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMEH305 HA NLS SpCas9 3x high affinity MS2 loops
Plasmid#168216PurposeMammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tagDepositorInsertSV40 NLS SpCas9
UseLentiviralTagsHA, SV40 NLS, and nucleoplasmin NLSExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7pr-His6-MBP-TEV-dCas9-BirA*
Plasmid#159994PurposeBacterial expression of dCas9-BirA*DepositorInsertdCas9-BirA*
Tags6X-His, MBP, 3X-FLAG, NLSExpressionBacterialMutationD10A, H840APromoterT7Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963
Plasmid#134612PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
TagsHaloExpressionMammalianMutationWT with deletion of the 375 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBV-luc TOP2A 1kb promoter
Plasmid#138274PurposeLuciferase reporter plasmid driven by TOP2A promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOP2A promoter sequence
UseLuciferaseExpressionMammalianAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.FRB-3myc-GS-KIF3A tail 419-702
Plasmid#134614PurposeMammalian expression of human KIF3A 419-702DepositorInsertKIF3A (KIF3A Human)
TagsFRB, 3mycExpressionMammalianMutationWT with deletion of the 418 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only