We narrowed to 16,540 results for: puromycin

1. AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

   Plasmid

   #184395

   Purpose

   Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Firefly luciferase beta-globin
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Promoter

   Tet-On 3G

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCMV-H2B-FT-Medium

   Plasmid

   #157670

   Purpose

   Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

   Depositor

   Shangqin Guo

   Article

   Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

   Insert

   Histone H2B
   

   Use

   Retroviral

   Tags

   Medium-FT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LEPZ

   Plasmid

   #111161

   Purpose

   miR-E (miR-30 variant)-based RNAi with ZsGreen reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Mutation

   WT

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

   Plasmid

   #176310

   Purpose

   Expresses CreN59-nMag-NLS with mCherry marker.

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   CreN59_nMag_NLS_P2A_mCherry_IRES_puroR
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human EF1alpha

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX459-TP53-exon5

   Plasmid

   #217456

   Purpose

   For TP53 knockout targeting exon 5 of TP53

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for TP53 (TP53 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1P shSOD1

   Plasmid

   #102975

   Purpose

   Suppression of SOD1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shSOD1 (SOD1 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA3379

   Plasmid

   #46874

   Purpose

   A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

   Depositor

   Mikhail Alexeyev, Troy Stevens

   Article

   Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

   Insert

   soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)
   

   Use

   Lentiviral

   Tags

   Myc

   Promoter

   TRE-Tight

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Barcode backbone

   Plasmid

   #158665

   Purpose

   This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   hcRED

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabePuro-ApoECDS

   Plasmid

   #42949

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

   Insert

   Apolipoprotein E (APOE Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pHAGE-3FLAG-RAB5A

    Plasmid

    #176488

    Purpose

    For lentiviral transduction of 3FLAG-RAB5A

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV-IFP2.0 T2A HO1

    Plasmid

    #59427

    Purpose

    Express infrared fluorescent protein IFP2.0 plus HO1 enzyme

    Depositor

    Xiaokun Shu

    Article

    Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

    Insert

    IFP2.0T2AHO1 IRES EGFP
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid

    #129668

    Purpose

    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-MSK1

    Plasmid

    #165601

    Purpose

    Expresses Sp dCas9 fused to human MSK1

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    ribosomal protein S6 kinase A5 (RPS6KA5 Synthetic, S. Pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Promoter

    TRE3G

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCKC014

    Plasmid

    #226623

    Purpose

    pLenti wt hTdT template for library 3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    Terminal deoxynucleotidyl transferase (DNTT Human)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

    Plasmid

    #85715

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV PIG Erg

    Plasmid

    #66984

    Purpose

    Retroviral expression plasmid for mouse Erg expression; containing bicistronic IRES-GFP

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Erg (Erg Mouse)
    

    Use

    Retroviral

    Promoter

    LTR

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMCB619

    Plasmid

    #171011

    Purpose

    CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    U6:sgRNA_Puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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