We narrowed to 10,611 results for: t7

1. pMJ841

   Plasmid

   #39318

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   T7

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. BSA1_His

   Plasmid

   #202766

   Purpose

   bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   BSA1
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-flag-mZscan4d-polyA

   Plasmid

   #61831

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Zscan4d (Zscan4d Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK194

   Plasmid

   #59563

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP XbaI attB1 Kozak mKate opal attB2 AscI
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-21a(+)-AK1-His

   Plasmid

   #118977

   Purpose

   Bacterial expression plasmid for Adenylate kinase isoenzyme 1

   Depositor

   Sebastian Maerkl

   Article

   Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

   Insert

   adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
   

   Tags

   6x-His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-smURFP-StagRFP-EKAR-EV

   Plasmid

   #173019

   Purpose

   Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   smURFP-StagRFP-EKAR-EV
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p11d-sctRPA

   Plasmid

   #102614

   Purpose

   Expression of S. cerevisiea RPA in E.coli

   Depositor

   Marc Wold

   Article

   Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

   Inserts

   RPA1 (RFA1 Budding Yeast)
   RPA2 (RFA2 Budding Yeast)
   RPA3 (RFA3 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   RFA2 has intron deleted

   Promoter

   T7

   Available Since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET14-GFP30_Encap

   Plasmid

   #86406

   Purpose

   Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 30aa CLP tag
   Encapsulin
   

   Tags

   6x Histidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM6-vioABECD

   Plasmid

   #66535

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Consensus T7

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPS22 (pET23a-Cas10/Csm/csm2H6N)

    Plasmid

    #70038

    Purpose

    Bacterial expression of the S. epidermidis Cas10·Csm complex

    Depositor

    Luciano Marraffini

    Article

    Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

    Insert

    Cas10/Csm complex
    

    Tags

    6xHIS on N terminus of Csm2

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a-4SS-HEWL

    Plasmid

    #39233

    Depositor

    Harald Schwalbe

    Article

    Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

    Insert

    Hen egg white lysozyme (LYZ Chicken)
    

    Expression

    Bacterial

    Mutation

    N-terminal Met

    Promoter

    T7

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET23 P53DBD

    Plasmid

    #174503

    Purpose

    express P53 DNA binding domain (94-312) in E.coli without tag

    Depositor

    Stewart Loh

    Article

    Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

    Insert

    P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
    

    Tags

    none

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-hXRCC1

    Plasmid

    #70759

    Purpose

    bacterial expression of human XRCC1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    XRCC1 (XRCC1 Human)
    

    Tags

    6-HIS

    Expression

    Bacterial

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-Ec.coaBC (pCLK1210)

    Plasmid

    #50448

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

    Insert

    PPCS PPCDC
    

    Tags

    6XHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1m-ELP

    Plasmid

    #170763

    Purpose

    EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    EF1m-ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSETa_ mEos4b

    Plasmid

    #51073

    Purpose

    Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

    Depositor

    Loren Looger

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    mEos4b
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET30-RT1306

    Plasmid

    #131521

    Purpose

    Expresses engineered RT-1306 with His-tag

    Depositor

    Bryan Dickinson

    Article

    Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

    Insert

    RT-1306
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-2(KW)2-eGFP

    Plasmid

    #112736

    Purpose

    Express 2(KW)2-eGFP in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-eGFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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