We narrowed to 10,047 results for: yeast

1. pILGFP5EA4

   Plasmid

   #185879

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFP5EC4

   Plasmid

   #185880

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFP5EF3

   Plasmid

   #185881

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pILGFP3AE4

   Plasmid

   #185873

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pREP41-MCS+

   Plasmid

   #52690

   Purpose

   Same as pREP41, with better MCS

   Depositor

   Michael Nick Boddy

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pnmt41

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS12-mCherry

   Plasmid

   #25444

   Depositor

   Benjamin Glick

   Insert

   mCherry
   

   Use

   Tags

   COX4 Presequence

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREP42-MCS+

   Plasmid

   #52691

   Purpose

   Same as pREP42, with better MCS

   Depositor

   Michael Nick Boddy

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pnmt41

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pILGFP5EG3

   Plasmid

   #185878

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pILGFP3A5C

   Plasmid

   #185872

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP6G-ccdB

    Plasmid

    #83880

    Purpose

    Destination vector for integration at yeast URA3 locus, expression from the GPD promoter

    Depositor

    Jennifer Nemhauser

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP4G-cccdB

    Plasmid

    #83876

    Purpose

    Destination vector for integration at yeast Trp locus, expression from the GPD promoter

    Depositor

    Jennifer Nemhauser

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP5G-ccdB

    Plasmid

    #83879

    Purpose

    Destination vector for integration at yeast Leu locus, expression from the GPD promoter

    Depositor

    Jennifer Nemhauser

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-FLAG-Rheb

    Plasmid

    #19996

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETCON-PTX-S1-220

    Plasmid

    #67747

    Purpose

    Pertussis Toxin Subunit 1 Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    PTxS1-220
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #78231

    Purpose

    Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-mCherry-FLAG-CD59-GPI

    Plasmid

    #50378

    Purpose

    Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

    Depositor

    Reika Watanabe

    Article

    Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only