We narrowed to 9,848 results for: EPO

1. pErCas12a EZ Clone

   Plasmid

   #132641

   Purpose

   Expresses ErCas12a and an sgRNA to target a region of interest

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   AAV and CRISPR

   Tags

   3x HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Q926R (Please see depositor comments) and BsaI si…

   Promoter

   CMV Promoter

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiPGK Puro DEST p38KTRClover

   Plasmid

   #59152

   Purpose

   Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2241

   Plasmid

   #111896

   Purpose

   ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple320 (POU4F1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ple320 (POU4F1 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1303 DV-SFTPC-tdTomato

   Plasmid

   #126696

   Purpose

   donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

   Plasmid

   #223213

   Purpose

   Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   TGFb1 active - membrane (TGFB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFOXA2-T2A-mTagBFP2-PGK-Neo

   Plasmid

   #223191

   Purpose

   BFP reporter plasmid targeting the human FOXA2 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)
   

   Use

   Cre/Lox; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS2273

   Plasmid

   #111899

   Purpose

   ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple343 (TUBB3 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ple343 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV-Sox10MCS5-GFP-EF1a-mCherry

   Plasmid

   #115782

   Purpose

   Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Inserts

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   EF1alpha promoter driven constitutive mCherry
   

   Use

   Lentiviral

   Tags

   EF1α promoter fused to mCherry and cfos promoter …

   Expression

   Mutation

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. hTph2p-Luc

   Plasmid

   #223526

   Purpose

   Firefly-luciferase reporter expression driven by human Tph2 promoter

   Depositor

   Kyungjin Kim

   Article

   Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

   Insert

   Tph2 promoter (TPH2 Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   hTph2 promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Psicheck Cdk2ap1Exon1

    Plasmid

    #178028

    Purpose

    expression vector to test translation efficiency by luciferase reporter.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Luciferase; Nonviral

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Cloned in

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-DIO-PSD95-EGFP-WPRE

    Plasmid

    #133785

    Purpose

    Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    PSD95-EGFP (Dlg4 Rat)
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Tag2B EDD

    Plasmid

    #37188

    Depositor

    Darren Saunders, Charles Watts

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Use

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    CMV

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. LSB-hsa-miR-17-5p

    Plasmid

    #103274

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-17-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-17-5p target (MIR17 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LSB-hsa-miR-17-3p

    Plasmid

    #103273

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-17-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-17-3p target (MIR17 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

    Plasmid

    #223273

    Purpose

    Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

    Plasmid

    #231007

    Purpose

    Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

    Insert

    E4F1 aa220-242 (E4F1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. LSB-hsa-miR-151a-3p

    Plasmid

    #103258

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-151a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-151a-3p target (MIR151A Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LSB-hsa-miR-151a-5p

    Plasmid

    #103259

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-151a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-151a-5p target (MIR151A Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCAR_sgRNA_hygro-tagBFP-lox5171

    Plasmid

    #162070

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TLCV2-RB1

    Plasmid

    #87836

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Insert

    sgRB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter and U6

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. SIN40C.TRE.ARID3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid

    #169314

    Purpose

    Doxycycline-inducible expression of human ARID3A cDNA with dTomato as reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    ARID3A (ARID3A Human)
    

    Use

    Lentiviral

    Tags

    IRES-dTomato

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

    Plasmid

    #218793

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    NLSf-mScarletP2A-hLuc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSCAR_sgRNA_blast-tagBFP-lox5171

    Plasmid

    #162072

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EGFPC2-Gephyrin P1

    Plasmid

    #68815

    Purpose

    expression of fluorescent tagged Geph in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyri…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSET iGluSnFR3 v82

    Plasmid

    #175187

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v82
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEMHE-membrane-mEGFP

    Plasmid

    #105526

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    GNAI2 (GNAI2 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    fragment encoding the plasma membrane targeting s…

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

    Plasmid

    #223277

    Purpose

    Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3

    Plasmid

    #136959

    Purpose

    Reporter vector encoding firefly luciferase and TurboRFP fluorescent protein

    Depositor

    Jakob Reiser

    Article

    Gills et al Oncotarget. 2018 Aug 24;9(66):32718-3272

    Insert

    Firefly luciferase and TurboRFP
    

    Use

    Lentiviral

    Tags

    V5 tag

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSCAR_sgRNA_hygro-tagBFP-lox2272

    Plasmid

    #162078

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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