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pSADdeltaG-FLPo-DsRedX

Plasmid

#32650

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

FLPo
DsRedX

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-kcnk3b

Plasmid

#208986

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk3b (kcnk3b Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBS Rax (CC#5)

Plasmid

#15138

Depositor

Connie Cepko

Article

Furukawa et al Proc Natl Acad Sci U S A. 1997 Apr 1. 94

Insert

Rax (Rax Mouse)

Expression

Bacterial

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
Lentihair s1GFP

Plasmid

#8451

Depositor

Bob Weinberg

Article

Stewart et al RNA 2003 Apr;9(4):493-501.

Insert

siRNA against GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pBN280

Plasmid

#86731

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Ptph-1::FLP D5

Expression

Worm

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC59-Halo

Plasmid

#175327

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC59 (CCDC59 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pXcX-3.6TPH-GAL4p65

Plasmid

#32573

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

Gal4p65

Use

Adenoviral

Expression

Mammalian

Promoter

3.6TPH

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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RSV CREB M1

Plasmid

#22395

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pBbS5a-RFP

Plasmid

#35283

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1

Plasmid

#21684

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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M4758 KanMX::URA3 Disruptor Converter

Plasmid

#51688

Purpose

Yeast marker swap plasmid for converting KanMX3 to URA3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

URA3 (URA3 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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Cas12c-sgRNA_BbsI_CAM

Plasmid

#183221

Purpose

Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA2a-RFP

Plasmid

#35325

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pHES841

Plasmid

#89194

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Expression

Yeast

Promoter

pZ

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-mCherry-Myc

Plasmid

#32637

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

mCherry

Use

Rabies virus

Tags

6xMyc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt10b

Plasmid

#42289

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt10b (Wnt10b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pBPHLWL-GAL80

Plasmid

#26257

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL80 (GAL80 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt8b

Plasmid

#42285

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8b (Wnt8b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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