We narrowed to 16,241 results for: LEA
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Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRExpressionMammalianAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hU6-cs1-PuroR-T2A-BFP
Plasmid#230934PurposegRNA cloning vector with direct capture sequence 1 in stem loop 2DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsTagBFPAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Tv9nL
Plasmid#188069PurposePROSS-designed high-redox potential laccase from Trametes versicolorDepositorInsertPROSS-designed high-redox potential laccase from Trametes versicolor
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation79 PROSS mutations, described in publicationAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
xCas9
Plasmid#138565PurposeExpresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSDDepositorInsertxCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ EB3-mKate2
Plasmid#105940Purposein vitro transcriptionDepositorAvailable SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1-U6abc_ect2_cmlc2-nmKate
Plasmid#238410PurposeDrives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytesDepositorInsertnuclear mKate/3 gRNAs targeting ect2
Promotercmlc2 (nmKate); U6 (gRNAs)Available SinceNov. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidExpressionMammalianPromoterHuman PGK1 gene promoterAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
CT691
Plasmid#233660PurposeBLADE (two-input, four-output decoder); Cre excises lox sites to express GFP, Flp excises FRT sites to express iRFP, and the presence of both recombinases is designed to express mRuby.DepositorInsertBLADE
ExpressionMammalianMutationNoneAvailable SinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-LAP2beta
Plasmid#175112PurposeLentiviral expression of V5-tagged mouse TmpoDepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sL7-Cre-HA-WPRE
Plasmid#204488PurposeFor production of driver AAV virus for Purkinje cell-specific expressionDepositorInsertCre
UseAAV, Cre/Lox, and Mouse TargetingExpressionMammalianPromotersL7Available SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEasiLV-MCS-coICP0
Plasmid#235545PurposeTo express ICP0 (doxycycline inducible)DepositorInsertICP0
UseLentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
HMBP-3C-STAR.66-285
Plasmid#100094Purposeexpresses MBP-tagged STARD1 protein domain (residues 66-285)DepositorAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts2
Plasmid#132714PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat NtsDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDarmo.CMVT_v1
Plasmid#133072PurposeEntry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-Flag-TwinStrep and TwinStrep-Flag-3CExpressionMammalianPromoterCMVT (Enhanced CMV)Available SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTM
Plasmid#162783PurposeProtein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are codedDepositorTypeEmpty backboneTags10xHis, c-myc, and hTPA leaderExpressionMammalianPromoterCHO Elongation factor 1AAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1
Plasmid#107407PurposeLentiviral expression of gRNA resistant PBRM1DepositorAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CKAR2
Plasmid#123348PurposeImproved FRET-based biosensor for monitoring Protein Kinase C activity.DepositorInsertCKAR2
Tags6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…ExpressionMammalianPromoterCMVAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNES-EGFP-C1-flex-PABD-Spo20
Plasmid#220081PurposeSpo20-PABD PA biosensor with a flexible linker to aid in nuclear exportDepositorInsertSpo20 (SPO20 Budding Yeast)
TagsX. leavis map2k1.L(32-44)-EGFP-SGGGSGGSExpressionMammalianMutationamino acids 51-91PromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only