We narrowed to 12,047 results for: LEA
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Plasmid#162786PurposeThe extracellular domain of Angiotensin converting enzyme 2 (ACE2) expressionDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
Tags10xHis, FLAG, and hTPA leaderExpressionMammalianPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
KRAS-P34R
Plasmid#158527PurposeExpresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialPromoterT5Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046286
Plasmid#149147PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDipA-TEV-His12
Plasmid#137042Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1200
Plasmid#138526PurposeFor expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.DepositorTagsN-terminal His tag and TEV cleavage site on MlaDExpressionBacterialPromoteraraBADAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin
Plasmid#133026PurposeExpresses EGFP-tagged tail anchor sequence of BikDepositorInsertBcl-2-interacting killer (BIK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-110PromoterHuman elongation factor 1 alphaAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB65 - pL0_tGFP-I (CDS1)
Plasmid#123182PurposeGolden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)DepositorInsertturboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
UsePart for plant expressionExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-gp160-754*
Plasmid#123274PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncationDepositorInsertHIV-1 (BG505) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutations; …PromoterCMVAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BaLgp140-8his
Plasmid#100924PurposeMammalian expression plasmid for soluble gp140 from the BaL HIV-1 strainDepositorInsertHIV-1 (BaL) Env
TagsCD5 leader sequence and Purification tag after D6…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_S212N
Plasmid#113951Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, S212N substitutionPromoterCMVAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAsub(TA)-TagRFPT-CAAX
Plasmid#87740PurposeNegative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).DepositorInsertPKAsub(TA)-TagRFPT-CAAX
Tags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mM-Asn209Ala_M18
Plasmid#91733PurposeExpression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
ExpressionMammalianMutationAsn209 changed to Ala (EU numbering)PromoterhEF1-HTLV promAvailable SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-CRY2-P14P5K
Plasmid#79569Purposeexpression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1cDepositorInsertPip5k1c (Pip5k1c Mouse)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationR445E and R446E; truncation of amino acids 636-66…PromoterCMVAvailable SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-RFP-UCR1C
Plasmid#67582PurposeExpresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cellsDepositorInsertC-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
TagsFLAG and TagRFPExpressionMammalianMutationAmino acid residues 80-136 of PDE4D3Available SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST47-CypB-Nucl2 C-term GFP
Plasmid#36126DepositorInsertcyclophilin B (PPIB Human)
TagsGFPExpressionMammalianMutationcontains CypB AA 34-211PromoterCMVAvailable SinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH371-SUP45-N58A
Plasmid#29378DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationN58A mutant gene including genomic sequence 1432 …Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBABEhygro-Flag-ZO-2-1st-PDZ-mut
Plasmid#27474DepositorInsertZonula Occludens 2 (TJP2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationK38E, F44H, I46E and V48EAvailable SinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2-delta-N
Plasmid#27419DepositorInsertZonula Occludens 2 (TJP2 Human)
Tags2xFlagExpressionMammalianMutationDeletion of coding nucleotides from 1-351. This r…Available SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRBC-PINBodyC-Tau(297-441)-WT-his6
Plasmid#253344PurposeExpress in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 297-441DepositorInsertTau 2N4R (MAPT Human)
TagsHis6 and PINBodyCExpressionBacterialMutationdeleted amino acids 1-296PromoterT7Available SinceMay 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pANAP-P2A-eRF(E55D)
Plasmid#241290PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factorDepositorInsertthe amber suppressor tRNACUA-Leu
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMVAvailable SinceDec. 11, 2025AvailabilityAcademic Institutions and Nonprofits only