We narrowed to 15,732 results for: LEA

1. pIS1 DICER1 long-mut-miR103/107

   Plasmid

   #21652

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant miR103/107 microRNA binding sites

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV Tmem53-V5

   Plasmid

   #175142

   Purpose

   Lentiviral expression of mouse Tmem53-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmem53 (Tmem53 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTox-EGFPsite1_NGA-PAM (BPK754)

   Plasmid

   #181747

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
   

   Use

   Selection plasmid

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL3

   Plasmid

   #184131

   Purpose

   For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.

   Depositor

   Alejandro Aballay

   Article

   Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

   Insert

   MRP-1 (mrp-1 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Pges-1 (3.3kb)

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXC4 (human) MSCV

   Plasmid

   #8546

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXC4 (HOXC4 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Contains improved ribosomal binding site and NcoI…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-HA-CUL1 L756A/I757A

   Plasmid

   #19938

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 L756A/I757A (CUL1 Human)
   

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   Mutation of two adjacent hydrophobic residues, L…

   Available Since

   April 17, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin

   Plasmid

   #134866

   Purpose

   Expresses EGFP-tagged mutant of VAMP1

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Vesicle-associated membrane protein 1 (VAMP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 97-118

   Promoter

   EF1a

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. XE223 human myc-Chibby-pCS2+MT

   Plasmid

   #16825

   Depositor

   Randall Moon

   Article

   Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

   Insert

   myc-Chibby (CBY1 Human)
   

   Use

   Xenopus expression

   Tags

   6-myc

   Available Since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pIS1 IMP-1 long-mut-let-7

   Plasmid

   #21642

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant let-7 microRNA binding sites

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG03

    Plasmid

    #73841

    Purpose

    Encodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.

    Depositor

    Jeffrey Tabor, Junghae Suh

    Article

    Gomez et al ACS Nano. 2015 Nov 30.

    Insert

    PhyB(1-917) with C-term his tag (PIL2 Mustard Weed)
    

    Use

    Dictyostelium discoideum expression

    Tags

    6x his tag

    Available Since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

11. PD-L1-miSFIT-6-C, 16-C

    Plasmid

    #124689

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MDH1-miR-196a-1-PGK-GFP-Blast

    Plasmid

    #31509

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a1 (Mir196a-1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid

    #84631

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ERKsub(TA)DD-Venus-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Mutated target Thr residue to Ala.

    Promoter

    CMV

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPC-N-Myc-Flag-BirA-hPOT1

    Plasmid

    #166409

    Purpose

    express BirA-hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Tags

    BirA, flag, and myc

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-DIO(ChRger1-TS-YFP)

    Plasmid

    #127245

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger1-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-DIO(ChRger3-TS-YFP)

    Plasmid

    #127242

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger3-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4/TO/myc-His-A-YAP2-deltaC

    Plasmid

    #21125

    Depositor

    Marius Sudol

    Article

    Tsutomu Oka & Marius et al

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Expression

    Mammalian

    Mutation

    5 Carboxy-terminal amino acids -FLTWL are deleted

    Available Since

    July 2, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

    Plasmid

    #166412

    Purpose

    express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 K90E (POT1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    EF-1a

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABEhygro-Flag-ZO-2-1st-PDZ-mut

    Plasmid

    #27474

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K38E, F44H, I46E and V48E

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only