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pCDNA3 mSmoothened NQ3

Plasmid

#126406

Purpose

Expression of Smoothened NQ3 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Mutation

Smo glycosylation site NQ3

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-neoR

Plasmid

#117048

Purpose

In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

neoR

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Tags

sfGFP

Expression

Bacterial

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-d80

Plasmid

#114025

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-d80

Plasmid

#114030

Purpose

Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-d80-HEC1 (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 1 to 80 deleted

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S15D

Plasmid

#114031

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S15D

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag D16A

Plasmid

#109031

Purpose

D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-fsaA

Plasmid

#102286

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

fsaA gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-adhE

Plasmid

#102290

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

adhE gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ompT

Plasmid

#102291

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

ompT gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRRG312-slc1a-5

Plasmid

#99102

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea slc1a-5 (also known as excitatory amino acid transporter)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

slc1a-5 (also known as excitatory amino acid transporter) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG94-runt-1

Plasmid

#99089

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Runt-1 (also known as runt)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

Runt-1 (also known as runt) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-srlA

Plasmid

#89965

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

srlA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rbsK

Plasmid

#89963

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rbsK gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-yhhT

Plasmid

#89958

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

yhhT gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-torZ

Plasmid

#89956

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

torZ gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-arsB

Plasmid

#89952

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

arsB gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSI HA Nup53xl

Plasmid

#86000

Purpose

mammalian expression of HA tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

HA

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSI myc Nup53xl

Plasmid

#86001

Purpose

mammalian expression of myc tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

myc

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-574-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85875

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-574-3p Sensor (MIR574 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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