We narrowed to 3,249 results for: cat.3

1. 5` CC: Puro – loxP – GFP-C

   Plasmid

   #219563

   Purpose

   5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   hPGK-promoter_PuroR_2A_loxP_GFP-C
   

   Use

   Unspecified

   Promoter

   hPGK

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLM-AMA15.0 Cas9

   Plasmid

   #138944

   Purpose

   Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

   Insert

   p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
   

   Use

   CRISPR and Synthetic Biology; Ama1 autonomously r…

   Tags

   NLS

   Promoter

   p40S (AN0465) Aspergillus nidulans

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMMP-MCS-IRES-eGFP-P2A-iCas9

   Plasmid

   #178533

   Purpose

   For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

   Depositor

   Ryoji Yao

   Article

   Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

   Insert

   iCaspase9 (CASP9 Human)
   

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-N-Myc_C223S USP7

   Plasmid

   #131243

   Purpose

   Mammalian expression of catalytically inactive Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 cyst…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCh-KIF17*-strep

   Plasmid

   #120167

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 17 (KIF17 Human)
   

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF17: 1-509 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDyn1

   Plasmid

   #64067

   Purpose

   Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).

   Depositor

   Andrew Carter

   Article

   Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

   Insert

   DYNC1H1 (DYNC1H1 Human)
   

   Use

   Cre/Lox

   Tags

   His-ZZ-TCS-SNAPf

   Expression

   Insect

   Mutation

   Codon optimized for Sf9

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTon1(il11)

   Plasmid

   #233647

   Purpose

   Expression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

   Depositor

   Takeo Kubo

   Article

   Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

   Inserts

   interleukin-11.L (350-913) (il11.L Frog)
   target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
   

   Use

   Amphibian expression, tet-on

   Mutation

   Some synonymous substitutions were introduced in…

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177345

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Synapsin

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAP4K3-GFP d344-873, K45E

   Plasmid

   #21735

   Depositor

   L. Miguel Martins

   Article

   Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

   Insert

   MAP4K3 (MAP4K3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of the carboxy-terminus, amino-acids 344…

   Available Since

   June 3, 2010

   Availability

   Academic Institutions and Nonprofits only

10. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid

    #177344

    Purpose

    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    dead hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3x GCN4 peptide, 3xHA, and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    Tetracycline-dependent promoters

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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