We narrowed to 15,830 results for: grna

1. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

   Plasmid

   #233035

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP712

   Plasmid

   #65768

   Purpose

   Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUG-natNT2

   Plasmid

   #110922

   Purpose

   expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   nat
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   AgTEF1

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPN089

   Plasmid

   #91614

   Purpose

   Express sgRNA targeting human FURIN

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   FURIN (FURIN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. POLR2A-N CRISPR pX330

   Plasmid

   #124495

   Purpose

   A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

   Depositor

   Masato Kanemaki

   Article

   Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

   Insert

   POLR2A targeting CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 promoter

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. BLADE-182

   Plasmid

   #134914

   Purpose

   sgRNA targeting GFP to be used in nanoblade system

   Depositor

   Théophile Ohlmann, Philippe Mangeot, Emiliano Ricci

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

   Plasmid

   #131508

   Purpose

   Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBC

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti-px330-CRBN-T1-pGK-Pur

   Plasmid

   #107382

   Purpose

   Mammalian expression CRISPR/Cas9

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   sgRNA targeting CRBN (CRBN Human)
   

   Use

   Lentiviral; Crispr

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV ORANGE Gria1-HaloTag KI

   Plasmid

   #139655

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE CACNG8-GFP KI #2

    Plasmid

    #131474

    Purpose

    Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacng8 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_BRD003 DLAT-1

    Plasmid

    #184484

    Purpose

    Lentivirus gRNA targeting human DLAT gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    DLAT-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_BRD003 DLAT-2

    Plasmid

    #184485

    Purpose

    Lentivirus gRNA targeting human DLAT gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    DLAT-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

    Plasmid

    #233039

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV_NLS-SaCas9-NLS-VPR

    Plasmid

    #68496

    Purpose

    AAV vector containing SaCas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SaCas9-VPR
    

    Use

    AAV and CRISPR

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE GFP-Clta KI

    Plasmid

    #131483

    Purpose

    Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Clta Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-Puro-sgSNRK

    Plasmid

    #138694

    Purpose

    Expresses a human SNRK-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSNRK human (SNRK Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJMP1333

    Plasmid

    #119268

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJMP1187

    Plasmid

    #119256

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiCRISPRv2 sgBLM10

    Plasmid

    #127643

    Purpose

    Knock-out of human BLM

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 sgRNA (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pORANGE Shank1-GFP KI

    Plasmid

    #131500

    Purpose

    Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Shank1, Shank1 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLC-BFP-FADD

    Plasmid

    #75166

    Purpose

    LentiCRISPR-BFP with sgRNA targeting human FADD

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    FADD sgRNA (FADD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pORANGE Rims2-GFP KI

    Plasmid

    #131495

    Purpose

    Endogenous tagging of RIM2: C-terminal (amino acid position: S1551)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rims2, Rims2 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. plenti-px330-CRBN-T2-pGK-Pur

    Plasmid

    #107383

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pORANGE Shank2-GFP KI

    Plasmid

    #131496

    Purpose

    Endogenous tagging of Shank2: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Shank2, Shank2 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSECC-sgTom

    Plasmid

    #138661

    Purpose

    Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pORANGE Doc2A-GFP KI

    Plasmid

    #131478

    Purpose

    Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Doc2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJMP1221

    Plasmid

    #119260

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDECKO_mCherry_non-targeting

    Plasmid

    #140109

    Purpose

    CRISPR-Cas9 library validation (negative control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJMP1104

    Plasmid

    #119248

    Purpose

    knockdown pqsC in P. aeruginosa

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA pqsC (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LentiGuide-Puro-IFITM3g1 (BB15)

    Plasmid

    #139460

    Purpose

    Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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