We narrowed to 10,611 results for: t7

1. pET-2(KW)2-mCherry

   Plasmid

   #112738

   Purpose

   Express 2(KW)2-mCherry in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-mCherry
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6-TEV-mCerulean3-pBluescript

   Plasmid

   #166757

   Purpose

   For purification of mCerulean3 fluorophore

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   mCerulean3
   

   Tags

   6x Histidine tag, and TEV protease site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET51b(+)_ALFA-tag-SNAP

   Plasmid

   #136628

   Purpose

   Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. human EB1 in PET28a for protein expression

   Plasmid

   #39297

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Article

   Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   6HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pET11a-Z-NGFP

   Plasmid

   #52734

   Purpose

   IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

   Depositor

   Lynne Regan

   Article

   Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

   Insert

   NGFP+Z-fusion
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. His6-TEV-Venus-pBluescript

   Plasmid

   #166756

   Purpose

   For purification of Venus fluorophore

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Venus
   

   Tags

   6x Histidine tag, and TEV protease site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pet42c(+)-GST-USP13

   Plasmid

   #61749

   Purpose

   express N-terminally GST tagged human USP13

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. untargeted Aurora B FRET sensor Kif2 substrate

   Plasmid

   #45215

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   untargeted Aurora B FRET sensor
   

   Tags

   CyPET, YPET

   Expression

   Mammalian

   Promoter

   t7

   Available Since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBz15_Strep_Rubisco

   Plasmid

   #140839

   Purpose

   wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   Form IA Rubisco; CbbL, CbbS
   

   Tags

   Strep II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET32a-hTGFa

    Plasmid

    #199236

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Transforming growth factor alpha, partial (TGFA Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mCherry-TSC2

    Plasmid

    #192440

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2 (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pALOD4 non-binding mutant

    Plasmid

    #111027

    Purpose

    Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A, G501A, T502A, T503A, L5…

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET16_Caspase-4_p10

    Plasmid

    #214308

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_Caspase-4_p20_C258A

    Plasmid

    #214307

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    C258A

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TAL2451

    Plasmid

    #36840

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HMGA2-TALEN_Right (Hmga2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. PT7-deGFP-MGapt

    Plasmid

    #67741

    Purpose

    T7 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T7term
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSW149

    Plasmid

    #122248

    Purpose

    pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

    Depositor

    Sarah Walker

    Article

    Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

    Insert

    TIF4631 (TIF4631 Budding Yeast)
    

    Tags

    Intein-Chitin-binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SAE1

    Plasmid

    #175241

    Purpose

    Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    SAE1 (SAE1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCellFree_G06_ZIKV E

    Plasmid

    #145351

    Purpose

    Cell-free expression vector for Zika virus Envelope protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Envelope
    

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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