We narrowed to 12,465 results for: cel.2;
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Plasmid#50881PurposeExpresses human NKCC1 N680C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationN680C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)
Plasmid#50864PurposeExpresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationA675C A734C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)
Plasmid#50869PurposeExpresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationS679C I730C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)
Plasmid#50863PurposeExpresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationI674C I730C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)
Plasmid#50868PurposeExpresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationI677C A734C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
GRM2-DuET
Plasmid#213301PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADRB2-DuET
Plasmid#213181PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AGTR2-DuET
Plasmid#213184PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTS-cox8A-GFP-IRES-mCherry
Plasmid#226103PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
TagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…Available SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-β2AR-μDpTT
Plasmid#182326PurposeBeta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertbeta2AR-μDronpa-TagRFP-T (ADRB2 Human)
TagsFull-length human beta2 adrenergic receptorExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRHR2-DuET
Plasmid#213216PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sPDGFRalpha-Fc
Plasmid#136438PurposeMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 FcDepositorInsertPDGFRalpha (PDGFRA Human)
TagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and L…ExpressionMammalianMutationExtracellular domain of PDGFR-alpha (a.a. 1-524)PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha-V242K
Plasmid#136436PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationV242K (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC(24)
Plasmid#124398PurposeFull-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
TagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624)Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flt3-Y591F/Y919F
Plasmid#74497PurposeOverexpression of FLT3 Y591F/Y919F mutant in mammalian cellsDepositorAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-LLAA(20)
Plasmid#124397PurposeFull-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
TagsFlagExpressionMammalianMutationL897A/L900A mutations (full length protein is 213…Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Brm BDmut-Flag pShuttle
Plasmid#122108PurposeExpression of full length Brm containing point mutations in its bromodomain in mammalian cellsDepositorAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha-Y206S
Plasmid#136435PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationY206S (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Repo-Man-1-890
Plasmid#184272PurposeExpresses C-terminally truncated GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
TagsGFPExpressionMammalianMutationtruncated Repo-Man after aminoacid 893PromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-S893D
Plasmid#204995PurposeExpresses shRNA resistant GFP-RepoMan S893D MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
TagsGFPExpressionMammalianMutationchanged Serine 893 to Aspartic acidPromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCA2-DuET
Plasmid#213308PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FPR2-DuET
Plasmid#213240PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sPDGFRalpha-Fc (Legacy)
Plasmid#136437PurposeMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 FcDepositorInsertPDGFRalpha (PDGFRA Human)
TagsFc region of IgG1 (a.a. Pro-217 to Lys-447) and L…ExpressionMammalianMutationExtracellular domain of PDGFR-alpha (a.a. 1-524)PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [Y252D]
Plasmid#126550PurposeExpresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationY252D point mutantPromoterLTRAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgSLC11A2
Plasmid#251684PurposegRNA to knock out SLC11A2 in mammalian cellsDepositorInsertSLC11A2 solute carrier family 11 member 2 (SLC11A2 Human)
UseCRISPR and LentiviralAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) K-Ras(G12C)
Plasmid#224284PurposeCell transfection and expression of K-Ras (G12C)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) K-Ras(WT)
Plasmid#224283PurposeCell transfection and expression of K-RasDepositorInsertK-Ras (KRAS Human)
ExpressionMammalianAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLY094-EFS-CD22-Blast-WPRE
Plasmid#192195PurposeLenti-CD22-OE-BlastDepositorAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY096-RetroEFS-CD22BBz-WPRE
Plasmid#192197PurposeRetro-CD22CARDepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY097-RetroEFS-CD22BBz-PRODH2-WPRE
Plasmid#192198PurposeRetro-CD22CAR-PRODH2DepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJV132
Plasmid#124254PurposeLentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 3DepositorAvailable SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP_AAVS1
Plasmid#141211PurposepDECKO_GFP_AAVS1_1801. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralExpressionMammalianAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_AAVS1_1801
Plasmid#159090Purposetargeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralExpressionMammalianAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-hNIL-BSD-mApple
Plasmid#124230PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PE2-IRES-ZeoR
Plasmid#207352PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.DepositorInsertPrime editor 2 (PE2) enzyme
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG-FGF13A
Plasmid#220793PurposeTo express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFPDepositorInsertFGF13 (FGF13 Human)
Available SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant
Plasmid#173963PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMinimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif
UseLuciferaseMutation1 mutated Coordinator motifPromoterSV40 promoterAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-EC1.45-human_min1-opossum_min2
Plasmid#173966PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseMutationSee Depositor Comments BelowPromoterSV40 promoterAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant
Plasmid#173962PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMinimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs
UseLuciferaseMutation4 mutated Coordinator motifsPromoterSV40 promoterAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only