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Flag-HRD1

Plasmid

#231688

Purpose

Expresses human HRD1 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

HRD1 (SYVN1 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2631

Plasmid

#144140

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

INSM1 (INSM1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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phGR-SB

Plasmid

#133409

Purpose

overexpression of a human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Expression

Mammalian

Mutation

wt

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Spop

Plasmid

#128872

Purpose

For mammalian expression of mouse Spop

Depositor

Aimin Liu

Article

Cai et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

Insert

Spop (Spop Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-CCSP:GFP

Plasmid

#68487

Purpose

Retrovirus expressing GFP driven by CCSP promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CCSP and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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p18S.1 tag

Plasmid

#51729

Purpose

transcribes 18S containing hybridization tag

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pReadthroughUAG

Plasmid

#11511

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, UAG stop codon, Renilla luciferase, and 3' UTR short RNA binding sites

Use

Luciferase

Available Since

March 15, 2006

Availability

Academic Institutions and Nonprofits only
pCAGGS-EGFP-SOS1

Plasmid

#178860

Purpose

Mammalian expression of SOS1 fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

SOS1 (SOS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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Lentiviral CaMKKbeta

Plasmid

#33322

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

Flag

Promoter

human PGK

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAdTrack-CMV HA ALAS-1

Plasmid

#22746

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

ALAS-1 (Alas1 Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1mLCAD_FLAG

Plasmid

#24484

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
evoBxb1_IVT

Plasmid

#222341

Purpose

Plasmid for evolved Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Use

Template for in vitro transcription

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0074

Plasmid

#142144

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

BACH2 (BACH2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomaGCaMP7

Plasmid

#182942

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-TCF7

Plasmid

#79498

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

TCF7 (TCF7 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Expression

Mammalian

Promoter

CAG

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pErbB2-ECFP

Plasmid

#66945

Purpose

Mammalian expression of rat ErbB2 tagged with ECFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

Insert

ErbB2 (Erbb2 Rat)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
ATF4 7: 5’3’ATF4.GFP

Plasmid

#21854

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Vector derived viral termination and poly-adenyla…

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF10wu

Plasmid

#149332

Purpose

Gateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF10wu (ORF10 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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