We narrowed to 8,358 results for: BLI

1. pBACgus4x/cdc37/LRRK2 1867-2186

   Plasmid

   #40364

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   HisTev

   Expression

   Mutation

   WT aa1867-2186

   Promoter

   polH

   Available since

   Oct. 2, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 4H4H

   Plasmid

   #51314

   Depositor

   Cheryl Arrowsmith

   Insert

   4H4H (EHMT1 4H4H, Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Truncation: See Comments

   Promoter

   T7

   Available since

   Feb. 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pFRT-TODestFLAGHA_HuD

   Plasmid

   #65757

   Purpose

   expresses FLAGHA-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hs.NRAS Q61L

   Plasmid

   #83178

   Purpose

   Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

   Depositor

   Dominic Esposito

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Q61L

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-trAtCCD8(pYL735)

   Plasmid

   #178286

   Purpose

   Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AICSDP-116:PCNA-mEGFP

   Plasmid

   #170818

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

   Depositor

   Allen Institute for Cell Science

   Insert

   PCNA Homology Arms with mEGFP-linker (PCNA Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pRN3P_Kdm5b_MUT

   Plasmid

   #119767

   Purpose

   in vitro transcription for mRNA

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   Kdm5b MUT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC28-hKdm2b-CxxC-PHD

   Plasmid

   #49216

   Purpose

   Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.

   Depositor

   Rob Klose

   Insert

   Kdm2b (KDM2B Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT-DestFLAGHA_HuD

   Plasmid

   #65760

   Purpose

   constitutively expresses FLAG-HA-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

10. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Use

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hs.HRAS Q61R

    Plasmid

    #83188

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61R

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MARS

    Plasmid

    #205232

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    

    Use

    Tags

    Nuclear Export Sequence and mScarlet-i

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT-TAK1-V5-His

    Plasmid

    #63753

    Purpose

    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    TGF-β activated kinase 1 (Tak1 Fly)
    

    Use

    Tags

    V5-His

    Expression

    Insect

    Mutation

    Promoter

    pMT

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid

    #41878

    Depositor

    Anna Huttenlocher

    Insert

    photoactivatable Rac1 (RAC1 Human)
    

    Use

    Zebrafish expression

    Tags

    6x His and mCherry

    Expression

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. DOT1L

    Plasmid

    #40736

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. K560GFP_17NL

    Plasmid

    #129767

    Purpose

    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    DAL insert before A345

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307-APN-G418

    Plasmid

    #158456

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDest eGFP CUGBP1

    Plasmid

    #61276

    Purpose

    Mammalian expression of EGFP-tagged human CUGBP1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    CUG-BP (CELF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-FURIN

    Plasmid

    #158442

    Purpose

    Gateway Cloning compatible entry vector for the human FURIN gene.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    FURIN (FURIN Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. NEK2

    Plasmid

    #39163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NEK2 (NEK2 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    T175A inactivating mutant

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only