We narrowed to 15,732 results for: LEA

1. pACB143

   Plasmid

   #222214

   Purpose

   For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   vanB(A24P)
   

   Tags

   N-terminal, thrombin-cleavable 6x His tag

   Expression

   Bacterial

   Mutation

   Changed Alanine 24 to Proline

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETSUMO2_Ideonella-bGSDM-WELQ

   Plasmid

   #222098

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Ideonella-bGSDM-WELQ ORF
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/T0-3XFLAG-NESm-NFATC2IP

   Plasmid

   #221445

   Purpose

   Mammalian expression vector of triple Flag tagged NFATC2IP ORF with mutant nuclear export signal (NESm) at N-terminus

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Tags

   3XFlag and Mutant Nuclear Export Signal (mNES)

   Expression

   Mammalian

   Mutation

   mutant NES (NESm: NLVDLQKKLEEAKADEQQ (PMID: 10739…

   Promoter

   CMV

   Available Since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pILGFPEasy9R

   Plasmid

   #218291

   Purpose

   Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP-SUN1ΔN

   Plasmid

   #213508

   Purpose

   Expresses human eGFP-SUNΔN in mammalian cells

   Depositor

   Karim Mekhail

   Article

   Shokrollahi et al bioRxiv 2023.05.07.539750

   Insert

   eGFP-SUNΔN
   

   Expression

   Mammalian

   Mutation

   SUN1ΔN has the first 138 amino acid (lamina domai…

   Promoter

   CMV

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP-43 mRuby K84ONBK

   Plasmid

   #216226

   Purpose

   TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   Transactive response DNA binding protein of 43 kDa (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Lysine 84 mutated to a TAG stop codon

   Promoter

   CMV Promoter

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCL1318

   Plasmid

   #217960

   Purpose

   Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   Histone H4 (H4C1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   L63A, F66A, I71A

   Promoter

   CMV

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCL1317

   Plasmid

   #217959

   Purpose

   Expression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   Histone H4 (H4C1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   GGG insertion between V65 and F66

   Promoter

   CMV

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCL1315

   Plasmid

   #217957

   Purpose

   Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   Histone H3.1 (H3C1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   F84A, L92A, Y99A

   Promoter

   CMV

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCL1314

    Plasmid

    #217956

    Purpose

    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    GGG insertion between A95 and C96

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAT_M4_YVTM_YQNQ

    Plasmid

    #197084

    Purpose

    This plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs.
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAT_M10_PQQAT

    Plasmid

    #197093

    Purpose

    This plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif.
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAT_M11_PQEINF

    Plasmid

    #197094

    Purpose

    This plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif.
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAT_M12_RPTAVEG

    Plasmid

    #197095

    Purpose

    This plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif.
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMAT_M13_6xSAG

    Plasmid

    #197096

    Purpose

    This plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_C

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    Control peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTY186 EGFP-T2A-ALFA-ORF6 SARS-CoV-2

    Plasmid

    #204976

    Purpose

    Co-express EGFP and SARS-CoV-2 ORF6 N-terminally labeled with ALFA tag

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-2)
    

    Tags

    ALFA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF702

    Plasmid

    #209650

    Purpose

    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

    Depositor

    Bastian Molitor

    Article

    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

    Inserts

    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-human-pro-IL-18_V47N_I48N

    Plasmid

    #214319

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myc

    Mutation

    V47N_I48N

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-human-pro-IL-18_LES33AAA

    Plasmid

    #214321

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myc

    Mutation

    LES33AAA

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-human-pro-IL-18_DDEN29ENGD

    Plasmid

    #214320

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myc

    Mutation

    DDEN29ENGD

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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