We narrowed to 9,414 results for: pho;

1. pEC3006 (pSpy1C-PZn_ffluc)

   Plasmid

   #218515

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PZn (PczcD)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CIB81-pmGFP

   Plasmid

   #75366

   Purpose

   Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Tags

   prenylated EGFP

   Expression

   Mammalian

   Mutation

   residues 1-81 of CIB1

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRetroX-Tight-MCS_PGK-OgNLuc

   Plasmid

   #70186

   Purpose

   Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-JEDI-1P-WPRE

   Plasmid

   #202613

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGI3EM17

   Plasmid

   #210569

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Promoter

   SpH2Bpro

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNAintron-XKR8-n3xFLAG

   Plasmid

   #215693

   Purpose

   Produces the XKR8 lipid scramblase with an n-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

   Insert

   XKR8-n3xFLAG (XKR8 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pSNRW-Z5-Nluc

   Plasmid

   #174487

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z5-Nluc
   

   Tags

   6x Histidine tag (N terminal on insert)

   Expression

   Bacterial

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-REPORT(PGK/CMV)-GRE

   Plasmid

   #172336

   Purpose

   Gal4 Response element

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   PGK/CMV

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pARF6(T27N)-CFP

   Plasmid

   #11386

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 6 (ARF6 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 27 to Asparagine.

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5HT6-YFP-Inp54p(D281A)

    Plasmid

    #96813

    Purpose

    phosphatase-dead control for 5HT6-YFP-Inp54p

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-Inp54p(D281A)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    D281A inactivates phosphatase activity

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PA-Cdc42

    Plasmid

    #75263

    Purpose

    Light-activated Cdc42

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    Cell Division Cycle 42 (CDC42 Human)
    

    Tags

    6xHIs, LOV2, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    F56W

    Promoter

    CMV

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZac2.1 GfaABC1D rM3D mCherry SV40

    Plasmid

    #92285

    Purpose

    AAV DREADD expression

    Depositor

    Baljit Khakh

    Article

    Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

    Insert

    rM3D
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    GfaABC1D

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-mEGFP(A206K)-paCaMKII-ShadowG

    Plasmid

    #167475

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII-ShadowG
    

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

    Plasmid

    #116885

    Purpose

    FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

    Depositor

    Brian Kuhlman

    Article

    Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

    Insert

    LINXa4(RVH)-Bre1
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

    Promoter

    ADH1

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-AgdNK

    Plasmid

    #203226

    Purpose

    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-FKBP-PI4KAC1001(D1957A)

    Plasmid

    #139312

    Purpose

    Recruitable PI4KA kinase-dead catalytic fragment

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
    

    Expression

    Mammalian

    Mutation

    D1957A, G1986D

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mTagBFP2-CD81-10

    Plasmid

    #55281

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    CD81 (CD81 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pPBAD-yhjH

    Plasmid

    #111613

    Purpose

    Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

    Depositor

    Tim Tolker-Nielsen

    Article

    Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

    Insert

    yhjH
    

    Expression

    Bacterial

    Mutation

    A52V

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

    Plasmid

    #116884

    Purpose

    FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

    Depositor

    Brian Kuhlman

    Article

    Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

    Insert

    LINXa4(RLR)-Bre1
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

    Promoter

    ADH1

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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