We narrowed to 9,259 results for: pho;

1. p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

   Plasmid

   #129650

   Purpose

   Rieske FeS overexpression

   Depositor

   Susanne von Caemmerer

   Article

   Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

   Insert

   CDS of PetC gene encoding for the Rieske FeS subunit
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Codon modified for Golden Gate

   Promoter

   pZmUbi

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a Gx-NL

   Plasmid

   #169105

   Purpose

   Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-mCherry.3xFLAG-WPRE

   Plasmid

   #127861

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   mCherry
   

   Use

   AAV

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-17b_Unc104-KIF5B-HALO

   Plasmid

   #169996

   Purpose

   Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

   Depositor

   Ron Vale

   Article

   Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

   Insert

   Kinesin-like protein Unc-104 fused with KIF5B
   

   Use

   Tags

   HALO-tag, 6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJR2956

   Plasmid

   #122533

   Purpose

   Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_CemOrange2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pnhx-2

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIG-Flag-RIPK1-wt

   Plasmid

   #102677

   Purpose

   retroviral expression plasmid for RIPK1-wt

   Depositor

   Hasam Habelhah

   Article

   Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   unknown

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTagBFP2-Dectin1A-C-10

   Plasmid

   #55290

   Purpose

   Localization: Membrane, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Dectin1A (CLEC7A Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pcDNA3-Lyn-sapphireAKAR

   Plasmid

   #118466

   Purpose

   cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-sapphireAKAR
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. XE49 pt Beta-catenin-myc

    Plasmid

    #16840

    Depositor

    Randall Moon

    Article

    Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

    Insert

    pt beta-catenin-myc (ctnnb1.L Frog)
    

    Use

    Xenopus expression

    Tags

    6x myc

    Expression

    Mutation

    four point mutations introduced into the GSK-3 ph…

    Promoter

    Available since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

12. SFG.CNa12_opt.IRES.eGFP

    Plasmid

    #22490

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin A mutant 12 (PPP3CA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    T351E; L354A

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. Twitch-2C pcDNA3

    Plasmid

    #104501

    Purpose

    fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]

    Plasmid

    #66781

    Purpose

    Gly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOG

    Depositor

    Yishi Jin

    Article

    Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    miniSOG
    

    Use

    Tags

    tomm-20 (N terminal 55 aa)

    Expression

    Worm

    Mutation

    G426C

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CapChR2_pUAST-attb

    Plasmid

    #188041

    Purpose

    Expresses CapChR2 in Drosophila

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2
    

    Use

    Tags

    none

    Expression

    Insect

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    UAS

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2

    Plasmid

    #191751

    Purpose

    Microtubule marker together with nuclear envelope marker driven by bidirectional promoter

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Inserts

    NCU04288 (NCU04288 Neurospora crassa)
    bml (Bml Neurospora crassa)
    

    Use

    Tags

    Gdp1 promoter, ToxA promoter, iRFP670, and mTagBF…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. ELC-Fc

    Plasmid

    #8636

    Depositor

    Timothy Springer

    Article

    Manjunath et al J Clin Invest 2001 Sep;108(6):871-8.

    Insert

    CCL19 (CCL19 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pGBW-m4046810

    Plasmid

    #149251

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG

    Plasmid

    #167477

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG

    Plasmid

    #167476

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(T286A)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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