We narrowed to 58,550 results for: tra
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Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFP-x-10
Plasmid#140143PurposeEvaluating the impact of high sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
ExpressionBacterialMutationD37, I38, and N39 are changed to nonsense (stop) …PromoternoneAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFP-x-.008
Plasmid#140141PurposeEvaluating the impact of low sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
ExpressionBacterialMutationD37, I38, and N39 are changed to nonsense (stop) …Available SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH825-CEN-minRLuc/slowstaCFLuc
Plasmid#115370PurposeExpresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferaseDepositorInsertsGcn4 5'-UTR + Firefly Luciferase
Renilla luciferase (codon minimised)
ExpressionYeastMutationAll codons have been changed for the least favour…PromoterADH1 and TDH3Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-YJR129c
Plasmid#85118Purposebacterial expression of YJR129cDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Spt2-K166R-GFP
Plasmid#115573PurposeExpresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166DepositorInsertSPT2
TagsGFPExpressionBacterial and YeastMutationLysine 166 mutated to ArginineAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-X2
Plasmid#115567PurposeGFP tethered to two repeats of the Gcn5 consensus motifDepositorInsertGreen Fluorescent Protein
ExpressionBacterial and YeastMutationTwo repeats of the Gcn5 consensus motif are tagge…PromoterADH1Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-X1
Plasmid#115566PurposeGFP tethered to a single repeat of the Gcn5 consensus motifDepositorInsertGreen Fluorescent Protein
ExpressionBacterial and YeastMutationSingle Gcn5 consensus motif repeat tethered to C-…PromoterADH1Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPI467
Plasmid#113208Purposeintegrating expression vector, C-terminal mScarlet taggingDepositorTypeEmpty backboneUseDictyostelium discoideum integrating expression v…TagsmScarletAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPI468
Plasmid#113207Purposeintegrating expression vector, N-terminal mScarlet taggingDepositorTypeEmpty backboneUseDictyostelium discoideum integrating expression v…TagsmScarletAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AX44_pS.Donor.S1
Plasmid#100289PurposeAAVS1-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingExpressionMammalianAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfA
Plasmid#89616PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG inductionDepositorInsertElectron transport flavoprotein alpha subunit
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfB
Plasmid#89617PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG inductionDepositorInsertelectron-transfer-flavoprotein, alpha polypeptide
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVH004
Plasmid#80401PurposeContains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.DepositorUseSynthetic BiologyTagsC-terminal LZx domainExpressionBacterialAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-lin26
Plasmid#80357PurposePmex-5:GFP::H2B::PEST::lin-26 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::lin-26 3'UTR
ExpressionWormAvailable SinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-atg4.2
Plasmid#80352PurposePmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::atg4.2 3'UTR
ExpressionWormAvailable SinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-set6
Plasmid#80362PurposePmex-5:GFP::H2B::PEST::set-6 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::set-6 3'UTR
ExpressionWormAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-ets4
Plasmid#80355PurposePmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::ets-4 3'UTR
ExpressionWormAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mex3
Plasmid#80361PurposePmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::mex-3 3'UTR
ExpressionWormAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mbk2
Plasmid#80360PurposePmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::mbk-2 3'UTR
ExpressionWormAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only