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pRL055 pDESTattB_cryaa:Venus

Plasmid

#213509

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRL092 pattB_cryaa:Venus_MCS

Plasmid

#213513

Purpose

attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-Nprl2

Plasmid

#99709

Purpose

Mammalian expression of HA-Nprl2

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

Nprl2 (NPRL2 )

Use

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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HA Nprl3 pRK5

Plasmid

#46330

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl3 (NPRL3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pRluc-LC3G120A

Plasmid

#105003

Purpose

Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Glycine 120 mutated to alanine, prevents lipidati…

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 A

Plasmid

#11324

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation A from figure 4A of paper.

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pCEP4-PRL-3

Plasmid

#16618

Depositor

Bert Vogelstein

Article

Saha et al Science. 2001 Nov 9. 294(5545):1343-6.

Insert

PRL-3 (PTP4A3 Human)

Use

Tags

2x HA

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pRL-TK CXCR4 6x

Plasmid

#11308

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

6 bulged bind sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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hPRL3_1-169

Plasmid

#209796

Purpose

Bacterial expression of human PRL-3 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2004 Mar 19. 279(12):11882-

Insert

PRL-3 (PTP4A3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 B

Plasmid

#11325

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation B from figure 4A of paper.

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
mutpRL-TK 3xUTR

Plasmid

#40758

Purpose

To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

3 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Expression

Mutation

mutated from TAG to CTC at nt 387–389 of the Reni…

Promoter

TK

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pRL-TK CXCR4 4x

Plasmid

#11307

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

4 bulged bind sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pRL-TK CXCR4 1P

Plasmid

#11309

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

1 perfect bind site for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-PRLH

Plasmid

#184618

Purpose

For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PRLH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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prLCA-Citrine

Plasmid

#40271

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

Clta (Clta Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pRLCon1-3180

Plasmid

#31445

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR (long) (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 D

Plasmid

#11327

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation D from figure 4A of paper.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
phpRL-BCL2-FL-pA

Plasmid

#42595

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL-TK let7 C

Plasmid

#11326

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation C from figure 4A of paper.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only