We narrowed to 383 results for: PEF1

1. pEF1α-ancestral-retroCHMP3-HA

   Plasmid

   #154248

   Purpose

   expresses ancestral New World monkey retroCHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed ancestral sequence of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF1-puro/hpb1

   Plasmid

   #59976

   Purpose

   integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression

   Depositor

   Timothy Springer

   Insert

   human integrin b1 headpiece (ITGB1 Human)
   

   Use

   Tags

   Base coiled-coil and His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a promoter

   Available since

   Sept. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pEF1a-CRY2PHR-SID4X

   Plasmid

   #63663

   Purpose

   AAV expression of CRYS2PHR-SID4X

   Depositor

   Dong Kong

   Article

   Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

   Insert

   SID4X-CYR2PHR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEF1a-mXRCC1pd-eGFP

   Plasmid

   #206035

   Purpose

   Mammalian expression of PAR-deficient mXRCC1pd coupled to eGFP under the control of a EF1a promoter

   Depositor

   Primo Schaer

   Article

   Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

   Insert

   mXRCC1pd
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S105A, S186A, K188A, S195A, S221A, S222A,S238A, S…

   Promoter

   Ef1a

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. vYM004_pNeo_phiC31_nTRE_pEF1s(orig)_citrine

   Plasmid

   #197677

   Purpose

   "pEF1s(orig)" reporter plasmid

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   pEF1s-H2B-Citrine
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF1α -NWM-Node 1

   Plasmid

   #154253

   Purpose

   expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed Node 1 of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF1α -NWM-Node 2

   Plasmid

   #154254

   Purpose

   expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed Node 2 of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF1α -NWM-Node 3

   Plasmid

   #154255

   Purpose

   expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed Node 3 of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF1a-PEmax-P2A-GFP

   Plasmid

   #202000

   Purpose

   Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

   Depositor

   Daniel Geschwind

   Insert

   PEmax-P2A-GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-pEF1a-EBFP-tTAn

    Plasmid

    #172122

    Purpose

    Constitutive expression of EBFP and rtTA-InteinN

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    EBFP, tTAn-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRLNeo-pEF1a-p53ash-mCerulean

    Plasmid

    #69579

    Purpose

    Expresses p53 tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

    Plasmid

    #160045

    Purpose

    For expressing the active auxin pump PIN2

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Insert

    PIN2
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    pEF1s

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP109 pEF1a-gRNA-GFP

    Plasmid

    #100935

    Purpose

    GFP, puromycin and gRNA scaffold driven by EF1a promoter

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA scaffold
    

    Use

    Grna scaffold

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRRLNeo-pEF1a-p53ashL344A-mCerulean

    Plasmid

    #69580

    Purpose

    Expresses p53-L344A mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF1α-Cre-PGK-Puro

    Plasmid

    #207742

    Purpose

    Co-expression of the Cre recombinase and a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pEF1α-squirrel-monkey-retroCHMP3-HA

    Plasmid

    #154174

    Purpose

    expresses squirrel monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRRLNeo-pEF1a-p53ashL344P-mCerulean

    Plasmid

    #69581

    Purpose

    Expresses p53-L344P mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents oliogomerization…

    Promoter

    EF1alpha

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-pEF1a-DIO-tTAc

    Plasmid

    #172126

    Purpose

    Expression of InteinC-tTAC in Cre recombinase positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-tTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF1α-spider-monkey-retroCHMP3-HA

    Plasmid

    #154179

    Purpose

    expresses spider monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LLP250 pEF1a-TALE-SunTag

    Plasmid

    #100939

    Purpose

    Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF1α-owl-monkey-retroCHMP3-HA

    Plasmid

    #154249

    Purpose

    expresses owl monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF1a_ TET1 CXXC only

    Plasmid

    #124084

    Purpose

    Expression of CXXC domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - CXXC domain (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEF1-puro/hpb1(short)

    Plasmid

    #59973

    Purpose

    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

    Depositor

    Timothy Springer

    Insert

    human integrin b1 (short) (ITGB1 Human)
    

    Use

    Tags

    Base coiled-coil and His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a promoter

    Available since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

25. LLP251 pEF1a-UNC5C-4-SunTag

    Plasmid

    #100940

    Purpose

    TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE target sequence for UNC5C, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LLP245 pEF1a-TALE-DNMT3a-Wt

    Plasmid

    #100937

    Purpose

    Empty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domain

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    

    Use

    TALEN

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LLP338 pEF1a-DACH1-1-SunTag

    Plasmid

    #100942

    Purpose

    TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE target sequence for DACH1, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF1α-mouse-CHMP3(147)-HA

    Plasmid

    #154177

    Purpose

    expresses mouse CHMP3(147) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (Chmp3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 147

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LLP469 pEF1a-mCherry-EMPTY-gRNA

    Plasmid

    #100958

    Purpose

    mCherry driven by EF1a, empty gRNA scaffold driven by U6

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA empty backbone with RFP
    

    Use

    CRISPR

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

    Plasmid

    #69583

    Purpose

    Expresses p53 tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge