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We narrowed to 139 results for: PYE

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Showing: 61 - 90 of 139 results

  1. pYES2-STE2-1D4

    Plasmid
    #133424
    Purpose
    Mutant yeast STE2 coding sequence in vector for expression in yeast S. cerevisiae, with Gal1 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Yeast
    Mutation
    C59S, C252A
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133424-plasmid-map-sequence-id-267643

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  3. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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  4. pYES2-gRNA-hyg-MCS

    Plasmid
    #107734
    Purpose
    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.
    Depositor
    Sheng Yang
    Article
    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.
    Insert
    X-3 site gRNA expression cassettes
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    SNR52p
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    107734-plasmid-map-sequence-id-208539

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  5. pYES2/NTA/Leu-LegA7

    Plasmid
    #216518
    Purpose
    Expression of LegA7
    Depositor
    Ralph Isberg
    Insert
    Leg A7
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 WT
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216518-plasmid-map-sequence-id-430062

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  6. pYES2/NTA/Leu-LegA7Δank

    Plasmid
    #216519
    Purpose
    Expression of LegA7 ΔAnk
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔAnk
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 331-454
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216519-plasmid-map-sequence-id-430063

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  7. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid
    #216520
    Purpose
    Expression of LegA7 ΔAnk290
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔAnk290
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 290-454
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216520-plasmid-map-sequence-id-430064

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  8. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid
    #216521
    Purpose
    Expression of LegA7 ΔNAnk264
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔNAnk264
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 264-361
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216521-plasmid-map-sequence-id-430065

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  9. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid
    #216522
    Purpose
    Expression of LegA7 ΔNAnk290
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔNAnk290
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 290-361
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216522-plasmid-map-sequence-id-430066

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  21. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey

    Plasmid
    #238132
    Purpose
    This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238132-plasmid-map-sequence-id-476568

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  22. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster

    Plasmid
    #238131
    Purpose
    This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238131-plasmid-map-sequence-id-476564

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  25. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse

    Plasmid
    #238133
    Purpose
    This plasmid encodes the full-length Mouse (Mus musculus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (Tmem106b Mouse)
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238133-plasmid-map-sequence-id-476563

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  26. pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

    Plasmid
    #237479
    Purpose
    Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (TMEM106B Human)
    Use
    Lentiviral
    Tags
    P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    M210A + F213A
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    237479-plasmid-map-sequence-id-476565

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  27. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

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Showing: 61 - 90 of 139 results