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Plasmid#119115PurposeBacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)DepositorInsertSH3PXD2A-PX (1-126) (SH3PXD2A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMay 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SH3BP5
Plasmid#23579DepositorInsertSH3BP5 (SH3BP5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-mRFP
Plasmid#170983PurposeEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-WT-short-UBASH3A
Plasmid#192088PurposeMammalian expression of human wild-type short-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SH3BP5L
Plasmid#23566DepositorInsertSH3BP5L (SH3BP5L Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SH3BP4
Plasmid#23643DepositorInsertSH3BP4 (SH3BP4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-2300
Plasmid#170985PurposeEncodes sgRNA target 2300 and Tetracycline resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-WT-long-UBASH3A
Plasmid#192087PurposeMammalian expression of human wild-type long-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-sh3bgr
Plasmid#192727PurposeGateway entry vector encoding zebrafish sh3bgrDepositorInsertsh3bgr (zgc:100896 Zebrafish)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasH3En2SE4P3Che(#1212)
Plasmid#184098Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#2
Plasmid#163401PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#3
Plasmid#163402PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#1
Plasmid#163400PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-SAE1_sh3
Plasmid#168985PurposeExpresses RFP cDNA and SAE1 shRNA 3 in mammalian cellsDepositorInsertSUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ shRNA-2
Plasmid#160068PurposeKnock Down SH3+ Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ 3m shRNA-1
Plasmid#160067Purpose3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGGcTGaTTaCAACAAGGATTG (mutations shown in lower c…Promotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ shRNA-1
Plasmid#160066PurposeKnock Down SH3+ Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA3
Plasmid#160213PurposeKnock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationTGTATGACCTCaGGcTGtACCA (mutations shown in lower …Promotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA2
Plasmid#160210PurposeKnock Down MTLSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA3
Plasmid#160212PurposeKnock Down MYDSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA2
Plasmid#160211PurposeKnock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationTGACGTTGCTCaGGcTGtACCA (mutations shown in lower …Promotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA1
Plasmid#160209PurposeKnock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…Promotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA1
Plasmid#160208PurposeKnock Down MQWSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF2-1/3-SV2/2
Plasmid#91408PurposeProtein expression and purification of human SH3 domain construct SH3RF2-1/3-SV2/2DepositorInsertSH3RF2-1/3-SV2/2 (SH3RF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF2-1/3-SV1/2
Plasmid#91322PurposeProtein expression and purification of human SH3 domain construct SH3RF2-1/3-SV1/2DepositorInsertSH3RF2-1/3-SV1/2 (SH3RF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-hSH3BP4
Plasmid#46895Purposeexpression of shRNA against human SH3BP4DepositorInsertSH3BP4 (SH3BP4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationW92A (mutated SH3 domain)PromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-mSH3BP4
Plasmid#46894Purposeexpression of shRNA against mouse SH3BP4DepositorInsertSH3BP4 (Sh3bp4 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationW92A (mutated SH3 domain)PromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFPC2-CB2 SH3-
Plasmid#68822PurposeEGFP tagged Collybistin expression in mammalian cellsDepositorInsertCollybistin (Arhgef9 Rat)
UseTagsEGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
WT-long-UBASH3A-V5-6xHis
Plasmid#192101PurposeMammalian expression of human wild-type long-form UBASH3A with V5-6xHis tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only