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pLV-NT-3-mCherry
Plasmid#190201PurposeTo express the mouse NT-3 tagged with the FP mCherryDepositorInsertNT-3
UseLentiviralTagsmCherryExpressionMammalianMutationThe codon were optimized for better expressionPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p401F/GgVcl 1-1066 (N term FLAG tag)
Plasmid#46284DepositorInsertVcl 1-1066 (VCL Chicken)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE8eWQ
Plasmid#161815PurposeExpresses ABE8eWQ in mammalian cellsDepositorInsertbpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-DBNF-mCherry
Plasmid#187195PurposeTo express the mouse BDNF tagged with the FP mCherryDepositorInsertBDNF
UseLentiviralTagsfluorescent protein mCherryExpressionMammalianMutationThe codons have been optimized for better express…PromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE-P48R
Plasmid#161816PurposeExpresses ABE-P48R in mammalian cellsDepositorInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
IMBB2.4-pUC57-mini
Plasmid#88845Purposeplasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)DepositorTypeEmpty backboneUseSynthetic Biology; CloningTagsExpressionMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE-P48R-UGI
Plasmid#161817PurposeExpresses ABE-P48R-UGI in mammalian cellsDepositorInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8r
Plasmid#208318PurposeOverxpression MBP-TadA8rDepositorInsertTadA8r
UseTagsExpressionBacterialMutationmutations in TadA are described in the paperPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
12XCSL-d1EGFP
Plasmid#47684PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsd1EGFPExpressionMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8e
Plasmid#208319PurposeOverxpression MBP-TadA8eDepositorInsertTadA8e
UseTagsExpressionBacterialMutationmutations in TadA are described in the paperPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
12XCSL-DsRedExpressDL
Plasmid#47683PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsdsRedExpressDRExpressionMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
MMTV-SV40-Bssk
Plasmid#1824DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
p18S.1 tag
Plasmid#51729Purposetranscribes 18S containing hybridization tagDepositorInsert18S
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
p18S.1 G693A
Plasmid#51728Purposetranscribes pactamycin resistant 18SDepositorInsert18S
UseTagsExpressionMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAND-MCS
Plasmid#223514PurposePlasmid backbone with multiple cloning site for two genes in opposing directions (convergently transcribed)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtac and PtetAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-Trp1
Plasmid#69196Purposecomplementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.DepositorInsertTRP1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-mNeonGreen
Plasmid#188248PurposeTemplate plasmid to transcribe mRNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-BDNF-pHluorin
Plasmid#187193PurposeTo express the mouse BDNF tagged with the FP pHluorinDepositorInsertBDNF (Bdnf Mouse)
UseLentiviralTagspPHluorinExpressionMammalianMutationThe codon were optimized for better expressionPromoterCMVAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTY-F
Plasmid#17439PurposeExpression of UTY in mammalian cellsDepositorInsertUTY (UTY Human)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
p18S.1 wt
Plasmid#51727Purposetranscribes wild type 18S without modificationsDepositorInsert18S
UseTagsExpressionMutationPromoterpol-IAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
p18S.2 tag
Plasmid#51731Purposetranscribes truncated transcript containing 18S and hybridization tagDepositorInsert18S
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w2-12-Oct1
Plasmid#18041DepositorInsertHD of Oct1
UseTagsExpressionBacterialMutationthe homeodomain of Oct1 is fused to fingers 1 and…PromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro.3xBsmBI
Plasmid#196709PurposeBackbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choiceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pTOPO-rDNA-NTS
Plasmid#23992DepositorInsertrDNA NTS
UseBacterial cloning - topoTagsExpressionMutationNonePromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-NanoLuc
Plasmid#188116PurposeTemplate plasmid to transcribe circular RNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-mNeonGreen
Plasmid#188115PurposeTemplate plasmid to transcribe circular RNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only
