Skip to main content
Addgene
Showing: 81 - 120 of 312 results

  3. pAW240

    Plasmid
    #170791
    Purpose
    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution
    Depositor
    Chang Liu
    Article
    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038
    Insert
    p10B2-AT110
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    10B2 (P1 specific promoter)
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170791-plasmid-map-sequence-id-339123

    Enlarge

  12. pAGK58

    Plasmid
    #228118
    Purpose
    Express node 814 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 814, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    228118-plasmid-map-sequence-id-458565

    Enlarge

  13. pAGK65

    Plasmid
    #228123
    Purpose
    Express node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228123-plasmid-map-sequence-id-455552

    Enlarge

  14. pAGK59

    Plasmid
    #228119
    Purpose
    Express node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228119-plasmid-map-sequence-id-455406

    Enlarge

  15. pAGK57

    Plasmid
    #228117
    Purpose
    Express node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228117-plasmid-map-sequence-id-455407

    Enlarge

  16. pAGK56

    Plasmid
    #228116
    Purpose
    Express node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 412, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228116-plasmid-map-sequence-id-455373

    Enlarge

  17. pAGK61

    Plasmid
    #228121
    Purpose
    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228121-plasmid-map-sequence-id-455410

    Enlarge

  18. pAGK60

    Plasmid
    #228120
    Purpose
    Express node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228120-plasmid-map-sequence-id-455404

    Enlarge

  19. pAGK62

    Plasmid
    #228122
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228122-plasmid-map-sequence-id-455403

    Enlarge

  20. pAGK55

    Plasmid
    #228114
    Purpose
    Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228114-plasmid-map-sequence-id-455303

    Enlarge

  22. pJBL708

    Plasmid
    #140378
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 10BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-10BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140378-plasmid-map-sequence-id-297838

    Enlarge

  27. pAW212

    Plasmid
    #172196
    Purpose
    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites
    Depositor
    Chang Liu
    Article
    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038
    Insert
    p10B2-AT110
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    10B2 (P1 specific promoter)
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172196-plasmid-map-sequence-id-338565

    Enlarge

  35. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid
    #220349
    Purpose
    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells
    Depositor
    Tomoko Igawa
    Article
    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.
    Inserts
    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    Use
    Tags
    Superman Repression Domain X (SRDX) and VP16 tran…
    Expression
    Plant
    Mutation
    two amino acid substitution in WUS-box: L255A L25…
    Promoter
    CaMV 35S and RPS5A
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220349-plasmid-map-sequence-id-440868

    Enlarge

  37. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid
    #170808
    Purpose
    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
    Depositor
    Kenneth Yamada
    Article
    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.
    Inserts
    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Synthetic, S. aureus, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Synonymous mutation in PYL1 to remove XhoI site (…
    Promoter
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170808-plasmid-map-sequence-id-334406

    Enlarge

  38. RPS1B-GFP (95)

    Plasmid
    #24039
    Depositor
    Michael Rout
    Insert
    RPS1B (RPS1B Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 31, 2010
    Availability
    Academic Institutions and Nonprofits only

  39. R777-E165 Hs.PIK3CB

    Plasmid
    #70449
    Purpose
    Gateway ORF clone of human PIK3CB [NM_006219.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3CB (PIK3CB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70449-plasmid-map-sequence-id-125898

    Enlarge

  40. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid
    #174893
    Purpose
    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette
    Depositor
    Simon Elsaesser
    Insert
    mCherry and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174893-plasmid-map-sequence-id-344146

    Enlarge

Showing: 81 - 120 of 312 results