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CUL5
Plasmid#38975PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCUL5 (CUL5 Human)
UseTagsTev His FlagExpressionBacterialMutationMouse Cul5 (GI:239051067) with two point mutation…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
GUCY1B3
Plasmid#39035PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGUCY1B3 (GUCY1B1 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3463
Plasmid#47182DepositorInsertZebrafishCommunity-Impacthomolog(mouse)-right
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3462
Plasmid#47181DepositorInsertZebrafishCommunity-Impacthomolog(mouse)-left
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PKM2
Plasmid#64698PurposeExpression of GFP tagged PKM2 in mammalian cellsDepositorInsertPKM2 (PKM Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
GUCY1B3
Plasmid#39121PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGUCY1B3 (GUCY1B1 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationThis is a complex of two proteins; GUCY1A3 (aa 46…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c011
Plasmid#175449PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.DepositorInsertCD44 (CD44 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_HUWE1_isoform1
Plasmid#190464PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS PKC theta
Plasmid#8426DepositorInsertMouse Protein Kinase C theta (Prkcq Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 22, 2005AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn PKM1
Plasmid#220920PurposeNeuronal specific expression of PKM1DepositorInsertPKM1 (PKM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shPKM2_4
Plasmid#42516DepositorInsertshRNA-PKM2 (PKM Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_R4187C
Plasmid#187141PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationR4187CPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_F3194S
Plasmid#187139PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationF3194SPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_H669Q
Plasmid#187137PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationH669QPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_H3962D
Plasmid#187130PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationH3962DPromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_HUWE1_3xFLAG
Plasmid#187155Purposemammalian cell expression of HUWE1-3xFLAGDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_C4341S
Plasmid#187156Purposemammalian cell expression FLAG-HUWE C4341SDepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationC4341SPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_FLAG-HUWE1
Plasmid#187121Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-WT
Plasmid#122690PurposeExpress His-tagged PKM2 (wild type) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
HUWE1-sgRNA-1
Plasmid#86924PurposeTo express sgRNA target HUWE1 geneDepositorInsertHUWE1 sgRNA1 (HUWE1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
HUWE1-sgRNA-2
Plasmid#86925PurposeTo express sgRNA target HUWE1 geneDepositorInsertHUWE1 sgRNA2 (HUWE1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human HUWE1 (3993-4374)
Plasmid#110754PurposeBacterial Expression of HUWE1 HECT domainDepositorInsertHUWE1 (HUWE1 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hPKM2
Plasmid#42515DepositorInsertPyruvate Kinase M2 (PKM Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_3xFLAG-HUWE1_R4187C
Plasmid#187142Purposemammalian cell expression of 3xFLAG-HUWE1 R4187CDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationR4187CPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-R399E
Plasmid#122691PurposeExpress His-tagged PKM2 (R399E) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationR399EPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-G415R
Plasmid#122693PurposeExpress His-tagged PKM2 (G415R) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationG415RPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_YH/GG
Plasmid#187123Purposemammalian cell expression of FLAG-HUWE1 Y355G/H356GDepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationY355G/H356GPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_3xFLAG-HUWE1_H3962D
Plasmid#187131Purposemammalian cell expression of 3xFLAG-HUWE1 H3962DDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationH3962DPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-H391Y
Plasmid#122692PurposeExpress His-tagged PKM2 (H391Y) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationH391YPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Kdr
Plasmid#48178Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of 2KDR (MC2R Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UIM-mut
Plasmid#187147PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationA1376G/S1382APromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_F3194S
Plasmid#187140Purposemammalian cell expression of FLAG-HUWE1 F3194SDepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationF3194SPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_3xFLAG-HUWE1_H669Q
Plasmid#187138Purposemammalian cell expression of 3xFLAG-HUWE1 H669QDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationH669QPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8-HUWE1-HECTonly
Plasmid#187119Purposeinsect cell expression of HUWE1 HECT domainDepositorInsertHUWE1 (HUWE1 Human)
UseTagsStrepExpressionInsectMutationdelta1-3992PromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA/UIM-mut
Plasmid#187151PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationM1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBM-mut
Plasmid#187149PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationL2976A/P2977APromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only
