We narrowed to 195 results for: 5113
-
Plasmid#170283PurposeTo monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInserta recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Mouse, Synthetic)
UseTagsmScarlet and mNeonGreenExpressionMammalianMutationPromoterAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HSPA5P
Plasmid#44150Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)DepositorAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PATagRFP-GRP78
Plasmid#238419PurposeExpresses GRP78 fused to the photoactivatable Tag-red fluorescent protein (PATagRFP-GRP78)DepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsPATagRFPExpressionMammalianMutationPromoterAvailable SinceSept. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-N Terminus (pGEX4T1)
Plasmid#238422PurposeExpresses GRP78 N terminus fused to the GST TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-C Terminus (pGEX4T1)
Plasmid#238423PurposeExpresses GRP78 C Terminus fused to the GST TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-T453D
Plasmid#235209PurposeExpresses GRP78 with T453D mutation in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsFLAGExpressionMammalianMutationChange Threonine 453 to Aspartic AcidPromoterAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-R197H
Plasmid#235208PurposeExpresses GRP78 with R197H mutation in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsFLAGExpressionMammalianMutationChange Arginine 197 to HistidinePromoterAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-G227D
Plasmid#235207PurposeExpresses GRP78 with G227D mutation in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsFLAGExpressionMammalianMutationChange Glycine 227 to Aspartic AcidPromoterAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-NLS-Mut
Plasmid#235206PurposeExpresses GRP78 with mutated NLS in mammalian cellsDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsFLAGExpressionMammalianMutationMutate the NLS sequencePromoterAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_TFAP2C_cterm
Plasmid#222916PurposeCas9/sgRNA plasmid for targeting TFAP2CDepositorAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2870
Plasmid#142165PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2871
Plasmid#143387PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-Full Length (pGEX4T1)
Plasmid#238421PurposeExpresses GRP78 Full Length fused to the GST TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
TFORF2363
Plasmid#142050PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-delta TM
Plasmid#100755PurposeMammalian expression of Nix dominant negativeDepositorAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-MaoB
Plasmid#100757PurposeMammalian expression of mitochondria targetted NixDepositorInsertNix-MaoB (BNIP3L Human)
UseTagsMycExpressionMammalianMutationTM domain replaced by MaoBPromoterCMVAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-CytoB5
Plasmid#100756PurposeMammalian expression of ER targeted NixDepositorInsertNix-CytoB5 (BNIP3L Human)
UseTagsMycExpressionMammalianMutationTM domain replaced by CytoBPromoterCMVAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-C73 (pGEX4T1)
Plasmid#238428PurposeExpresses GRP78 with the only the last 73 amino acids at the C terminus fused to the GST TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-Delta C17 (pGEX4T1)
Plasmid#238426PurposeExpresses GRP78 with the last 17 amino acids at the C terminus deleted fused to the GST TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-Delta C11(pGEX4T1)
Plasmid#238425PurposeExpresses GRP78 with the last 11 amino acids at the C terminus deleted fused to the GST TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-Delta C73 (pGEX4T1)
Plasmid#238427PurposeExpresses GRP78 with the last 73 amino acids at the C terminus deleted fused to the GST TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-Delta KDEL (pGEX4T1)
Plasmid#238424PurposeExpresses GRP78 Delta KDEL fused to the GST TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-B_sgRNA_POP1 (pAVA3497)
Plasmid#239311PurposeLentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting POP1DepositorInsertU6-driven sgRNA targeting POP1 (POP1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
NKP40 RFP (794)
Plasmid#62006Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF1330
Plasmid#143950PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Mito-Car-GECO
Plasmid#100765PurposeLentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imagingDepositorInsertLenti-Mito-Car-GECO
UseLentiviralTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1: E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G
Plasmid#204190PurposeDoxycycline inducible lentiviral vector for human TFAP2C overexpressionDepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP-GFP-shch-TOG
Plasmid#69113PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
IRAK4 gRNA (BRDN0001149331)
Plasmid#75666Purpose3rd generation lentiviral gRNA plasmid targeting human IRAK4DepositorInsertIRAK4 (IRAK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
IRAK4 gRNA (BRDN0001147550)
Plasmid#75665Purpose3rd generation lentiviral gRNA plasmid targeting human IRAK4DepositorInsertIRAK4 (IRAK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
IRAK4 gRNA (BRDN0001146161)
Plasmid#75664Purpose3rd generation lentiviral gRNA plasmid targeting human IRAK4DepositorInsertIRAK4 (IRAK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P-SSP-AAA Mutant
Plasmid#238432PurposeExpress amino acid 631-650 of AAA Mutant GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP)DepositorInsertamino acid 631-650 of AAA Mutant GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP) (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable SinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P-SSP-Wild-Type
Plasmid#238431PurposeExpress amino acid 631-650 of Wild-type GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP)DepositorInsertamino acid 631-650 of Wild-type GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP) (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable SinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-No UTRs Wild-Type (pcDNA3.1)
Plasmid#238429PurposeExpresses GRP78 Full Length without UTRs fused to the FLAG TagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsFLAGExpressionMammalianMutationThe UTRs of the gene have been deleted.PromoterAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HSPA5_WT
Plasmid#82132PurposeGateway Donor vector containing HSPA5 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
Plasmid#69114PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationL1939A and L1942A amino acid substitutions. Sile…PromoterCMVAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_IRAK4_WT
Plasmid#82286PurposeGateway Donor vector containing IRAK4, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GRP78-No UTRs AAA Mutant (pcDNA3.1)
Plasmid#238430PurposeExpresses GRP78 AAA mutant without the UTRs fused to FLAG tagDepositorInsert78-kDa glucose-regulated protein (HSPA5 Human)
UseTagsFLAGExpressionMammalianMutationThe UTRs of the gene have been deleted. The PPP t…PromoterAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDNA5-FRT/TO-FLAG-TONSL
Plasmid#221011Purposemammalian expression vector of Flag tagged TONSL siRNA resistantDepositorInsertTONSL (TONSL Human)
UseTagsFlagExpressionMammalianMutationsilent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…PromoterCMVAvailable SinceJune 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-IRAK4
Plasmid#23749DepositorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDNA5-FRT/TO-eGFP-TONSL
Plasmid#221012Purposemammalian expression vector of eGFP tagged TONSL siRNA resistantDepositorInsertTONSL (TONSL Human)
UseTagseGFPExpressionMammalianMutationsilent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…PromoterCMVAvailable SinceJune 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-FRT/TO-3xFLAG-REV7
Plasmid#114127PurposeExpresses N-terminally tagged 3x FLAG-REV7 in mammalian cellsDepositorInsertREV7 (MAD2L2 Human)
UseGenomic integration, tetracycline inducibleTags3xFLAGExpressionMammalianMutationPromoterCMV/TetO2Available SinceAug. 16, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-FRT/TO-eGFP-REV7
Plasmid#114128PurposeExpresses N-terminally tagged eGFP-REV7 in mammalian cellsDepositorInsertREV7 (MAD2L2 Human)
UseGenomic integration, tetracycline inducibleTagseGFPExpressionMammalianMutationPromoterCMV/TetO2Available SinceAug. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiT3.1-C_KCTD3:1-815
Plasmid#211286PurposeMammalian expression of full-length human KCTD3. C-terminal HiBiT tag.DepositorInsertKCTD3:M1-L815 (KCTD3 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_KCTD3:1-815
Plasmid#211212PurposeMammalian expression of full-length human KCTD3. N-terminal HiBiT tag.DepositorInsertKCTD3:M1-L815 (KCTD3 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVP65KR-gb-SacB(-)
Plasmid#171646PurposeEquipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.DepositorInsertMBP-mCherry-8xHis-SacB(-)
UseTags8xHisExpressionMutationinternal HindIII site in the SacB gene was remove…PromoterAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSADuet148
Plasmid#121910PurposeCharge-introduced NpuDnaBΔ283ΔC39 inteinDepositorInsertCI-NpuDnaBΔ290ΔC39 intein
UseTagsHis6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet259
Plasmid#121911PurposeNpuDnaBΔ283ΔC39 inteinDepositorInsertNpuDnaBΔ283ΔC39 intein
UseTagsHis6-GB1ExpressionBacterialMutationDeletion of 283-residue endonuclease domainPromoterT7Available SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBBAD113
Plasmid#121912PurposeCharge-Introduced NpuDnaBC39 inteinDepositorInsertCI-NpuDnaBC39 intein
UseTagsGB1-His6ExpressionBacterialMutationS110K, I111KPromoterParaAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only